C-erbB-2与c-raf-1基因反义寡核苷酸联合对人卵巢癌裸鼠皮下移植瘤的治疗作用  

Therapeutic Effects of C-erbB-2 and C-raf-1 Gene Combined with Antisense Oligodeoxynucleotide on the Human Ovarian Carcinoma Transplanted Subcutaneously in Nude Mice

作　　者：吴永忠[1] 任庆兰[2] 李少林[2] 

机构地区：[1]重庆医科大学附属第一医院肿瘤科,重庆400016 [2]重庆医科大学基础医学院核医学教研室,重庆400016

出　　处：《癌症》2003年第8期836-839,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金项目(No.30070230)

摘　　要：背景与目的:针对c-erbB-2与c-raf-1单基因的反义寡脱氧核苷酸(antisenseoligodexynucleotide,ASODN)治疗肿瘤的研究报道很多,这些研究往往局限于单个基因或细胞水平,从肿瘤发生的多基因学说上讲是不恰当的。本研究旨在探讨c-erbB-2与c-raf-1基因联合ASODN对人卵巢癌裸鼠皮下移植瘤的治疗作用。方法:在BALB/c裸鼠上建立卵巢上皮癌模型,然后随机分成阴性对照组和6个实验组(分别为脂质体c-erbB-2SODN组、脂质体c-raf-1SODN组、脂质体c-erbB-2ASODN组、脂质体c-raf-1ASODN组、全剂量联合反义给药组、半剂量联合反义给药组)。检测裸鼠体重及肿瘤体积变化,计算抑瘤率及肿瘤缩小率。结果:给药后,全剂量联合反义给药组与半剂量联合反义给药组的肿瘤体积抑瘤率分别为72.5%和78.4%,肿瘤重量抑瘤率分别为70.7%和75.3%,肿瘤缩小率分别为29.7%和41.6%。在给药后,各组裸鼠体重变化无明显统计学差别。结论:C-erbB-2与c-raf-1基因联合ASODN在体内能明显地抑制肿瘤生长。BACKGROUND &OBJECTIVE: Although many positive studies were reported on single C erbB 2 or C raf 1 antisense oligodeoxynucleo tide (ASODN) in cancer treatment, these studies were usually limited in single gene or in cell level and were not appropriate according to the multiple genes hypotheses of tumorigenesis. This study was designed to investigate the effects of C erbB 2 and C raf 1 combined with ASODN on the treatment of ovarian carcinoma xenograft in nude mice. METHODS: The model of xenografts derived from ovarian epithelial cancer SKOV3 cells was established in Balb/C nude mice, then they were randomly divided into a negative control group and 6 experimental groups Therapeutic Effects of C erbB 2 and C raf 1 Gene Combined with Antisense Oligodeoxynucleotide on the Human Ovarian Carcinoma Transplanted Subcutaneously in Nude Mice$$$$ Wu Yong Zhong1, Ren Qing Lan2, Li Shao Lin2 1.Department of Oncology,The First Affiliated Hospital,Chongqing Medical University,Chongqing, 400016,P.R.China 2.Department of Nuclear Medicine, Basic Medical College,Chongqing Medical University,Chongqing, 400016,P.R.China AbstractBACKGROUND &OBJECTIVE: Although many positive studies were reported on single C erbB 2 or C raf 1 antisense oligodeoxynucleo tide (ASODN) in cancer treatment, these studies were usually limited in single gene or in cell level and were not appropriate according to the multiple genes hypotheses of tumorigenesis. This study was designed to investigate the effects of C erbB 2 and C raf 1 combined with ASODN on the treatment of ovarian carcinoma xenograft in nude mice. METHODS: The model of xenografts derived from ovarian epithelial cancer SKOV3 cells was established in Balb/C nude mice, then they were randomly divided into a negative control group and 6 experimental groups [intraperitoneal injection of (1)liposome C erbB 2 ASODN, (2)liposome C raf 1 ASODN, (3)liposome C erbB 2 ASODN, (4)liposome C raf 1 ASODN, (5)whole dose combined ASODN, (6)half dose combin

关 键 词：C-ERBB-2 C-RAF-1 基因 反义寡核苷酸 卵巢癌 裸鼠 皮下移植瘤 治疗 

分 类 号：R737.31[医药卫生—肿瘤]