L-色氨酸生物合成的代谢流量分析  

Metabolic Flux Analysis of L-Tryptophan Biosynthesis

作　　者：王健[1] 陈宁[1] 张蓓[1] 谭青乔[1] 张克旭[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《微生物学报》2003年第4期473-480,共8页Acta Microbiologica Sinica

基　　金：天津市科委攻关项目 ( 983 1 1 3 71 1 )~~

摘　　要：建立了谷氨酸棒杆菌合成L 色氨酸 (L Try)的代谢流量平衡模型 ,应用该模型计算出发酵中后期的代谢流分布并通过MATLAB软件线性规划得到Try理想代谢流分布。结果表明 75 1 5 %的碳架进入糖酵解 ,2 4 85 %的碳架进入HMP途径 ;但与理想代谢流相比 ,应从遗传改造和发酵控制方面降低TCA循环的代谢流 ,减少副产氨基酸的生成 ,摸索最适的溶氧控制对提高Try产率至关重要。The metabolic flux balance model of L-Try synthesis by Corynebacterium glutamicum was constructed in this paper. Using this model, the metabolic flux distribution during the middle and late period were determined and the optimal flux distribution were calculated by linear program of MATLAB software. The analysis results indicate that 24.85% metabolic flux entered the HMP pathway and 75.15% entered the EMP cycle. But comparing to the optimal flux distributions, the production of L-Try should be improved from the genetic manipulation and fermentation control through reducing byproduct of amino acid and decreasing the metabolic flux of TCA and EMP.

关 键 词：L—色氨酸 生物合成 代谢流量 线性规划 发酵 

分 类 号：Q591[生物学—生物化学]