腺苷对体外培养的海马缺糖缺氧神经元的保护作用  

Protective effects of adenosine on cultured rat hippocampal neurons after oxygen-glucose deprivation

作　　者：丁爱石[1] 吴燕[1] 吴丽颖[1] 马子敏[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所神经生物学研究室,北京100850

出　　处：《中国病理生理杂志》2003年第8期1079-1083,共5页Chinese Journal of Pathophysiology

摘　　要：目的 :观察缺糖缺氧对大鼠海马培养神经元的影响及腺苷的保护作用。方法 :取培养 12d的海马神经元 ,分为对照组和腺苷组 ,同时于缺糖缺氧环境中培养 0 .5 - 4h后取出 ,立即更换原神经元培养液在常氧下继续培养 2 4h。于不同时间取出 ,收集培养液 ,测定培养液中乳酸脱氢酶 (LDH)含量 ,用图像分析仪测定神经元胞体面积 ,并用 4 %台盼蓝染色 ,计算神经元存活百分率。同时用原位末端标记 (TUNEL)法检测神经元凋亡。结果 :缺糖缺氧后海马神经元胞体肿胀 ,体积变大 ,LDH渗出含量增多 ,神经元存活率减少。恢复糖和氧供应后 2 4h ,海马神经元LDH渗出含量进一步增多 ,细胞存活率进一步减少 ,凋亡神经元百分率明显增多。经腺苷预处理的海马神经元缺糖缺氧后 ,神经元LDH渗出明显少于对照组 ,神经元胞体面积增大程度轻于对照组 ,神经元存活率明显高于对照组。缺糖缺氧 0 .5 - 3h再恢复氧和糖供应后 2 4h ,腺苷组凋亡神经元百分率亦明显低于对照组。结论 :缺糖缺氧可引起海马神经元严重损伤 ,神经元损伤程度与神经元存活率、LDH渗出含量的改变呈对应变化。腺苷能减少缺糖缺氧后神经元的损伤 ,减少缺糖缺氧后神经元凋亡 ,提示腺苷对缺糖缺氧海马神经元具有一定的保护作用。AIM:To investigate the protective effects of adenosine on cultured rat hippocampal neurons after oxygen-glucose deprivation. METHODS:The control and adenosine-treated hippocampal neurons cultured for 12 d were exposed to oxygen-glucose deprivation environment for 0.5-4 h and then cultured with original medium in normoxia for 24 h . The soma area,survival rate, effluxes of lactate dehydrogenase (LDH)and apoptosis of neurons were observed. RESULTS:The soma area, effluxes of lactate dehydrogenase from neurons and apoptosis were increased while survival rate of neurons was decreased after oxygen-glucose deprivation compared with those pre-oxygen-glucose deprivation. Compared with the control, after oxygen-glucose deprivation the soma area, effluxes of lactate dehydrogenase from neurons and apoptosis were decreased, however, the survival rate of neurons was increased in the adenosine group.CONCLUSION: Oxygen-glucose deprivation can lead to the severe damage of cultured hippocampal neurons, and adenosine can reduce neuronal injury induced by oxygen-glucose deprivation.

关 键 词：海马 腺苷 低糖 低氧 神经元 体外培养 保护作用 乳酸脱氢酶 细胞凋亡 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R338.26[医药卫生—临床医学]