肝细胞癌p53基因249密码子热点突变的研究  

Study of hotspot mutation of p53 gene codon 249 in hepatocellular carcinoma

作　　者：曾晖[1] 陆伟君[2] 杨玉秀[1] 曾毅 

机构地区：[1]河南省人民医院消化内科,郑州450003 [2]山东省立医院消化内科 [3]中国预防医科院病毒所肿瘤病毒室

出　　处：《胃肠病学和肝病学杂志》2003年第4期357-359,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：河南省科技攻关项目课题(001170231)

摘　　要：目的 研究肝细胞癌(HCC)中p53基因249密码子(p53 E7 cd249)点突变情况。方法 用PCR法及HAEⅢ限制性片段长度多态性分析(HAEⅢ/RFLP)检测河南豫东地区38例HCC石蜡包埋组织及2例肝细胞癌株中p53 E7cd249点突变情况,DNA测序证实。选取广西桂西南地区的10例HCC作对照。结果 来自河南豫东地区的HCC p53 E7 cd249点突变率为10.5％(4/38),对照组广西桂西南地区的HCC p53 E7 cd249点突变为40％(4/10),二者相比具有显著性差异(P<0.05)。2例肝细胞癌株中均未发现HCC p53 E7 cd249点突变。结论 河南豫东地区HCC中p53基因E7 cd249点突变为非高发事件;p53 E7 cd249点突变可能发生在肝细胞癌变的晚期。Objective To study the hotspot mutation of p53 codon 249 in hepatocellular carcinoma.Methods The paraffin-embedded tissues of 38 cases of HCC from Henan Yudong area as well as 2 kinds of hepatocellular carcinoma cell lines (HBV1,HBV2) were analysed for hotspot mutation of p53 codon 249 (p53 E7 cd249) using PCR and Restriction Fragment Length Polymorphism (PCR-RFLP). DNA-sequencing was done to clarify the result. 10 cases of HCC from Guangxi Guixinan area were selected for control. Results The frequency of p53 mutational hotspot were 10.5% (4/38) and 40% (4/10) respectively ( P <0.05) in two groups of HCC cases which came separately from Henan Yudong and Guangxi Guixinan. No p53 E7 cd249 point mutation were found in HBV1 and HBV2. Conclusions The frequency of hotspot mutation of p53 E7 cd249 in HCC from Henan Yudong area in lower and p53 E7 cd249 Point mutation is possibly happened at the later stage of hepatocellular carcinogenesis.

关 键 词：肝细胞癌 P53基因 249密码子 基因突变 乙型肝炎 基因多态性 

分 类 号：R735.7[医药卫生—肿瘤] R394[医药卫生—临床医学]