大肠癌及癌前病变的粘液组化研究  

Studies of Mucin Histochemistry in Colonic Carcinoma and Premalignant Lesions

作　　者：虞积耀[1] 王曾平 马述仕 王洪海[4] 杜毅[1] 夏敏良[1] 郑集义[1] 

机构地区：[1]解放军海军总医院病理科,北京100037 [2]北京军区总医院,100700 [3]解放军武警总医院,北京100039 [4]解放军空军总医院,北京100039

出　　处：《临床与实验病理学杂志》1992年第4期257-260,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：应用粘液组化方法AB—PAS、HID—AB、OR—AB、PAT—KOH—PAS、PATB—KOH—PAS显示大肠上皮分泌粘液的类型。结果发现:大肠慢性炎、大肠克隆病及炎性息肉、增生性息肉、幼年性息肉分泌粘液类型与正常粘膜相似(P>0.05),而伴有异型增生的溃疡性结肠炎、管状腺瘤、绒毛状腺瘤、家族性息肉、腺瘤癌变、大肠癌及癌旁粘膜粘液分泌性质与量均有变化。在这些病变中氮乙酰化及含有邻位羟基的氧乙酰化粘液阳性率明显高于正常粘膜(P<0.05)。其检出阳性率与异型增生的程度有关。笔者认为应用粘液组化方法分析大肠癌前病变粘液性质对于判断粘膜上皮恶性倾向提供了有价值的手段。The AB—PAS,HID—AB, OR—AB, PAT—KOH—PAS, PATB—KOH—PAS histochemical stains were applied to investigate the composition of mucin of colonic carcinoma and premalignant lesions. The results showed that similarity of mucin pattern in normal mucosa was observed in chronic inflammation, Crohn's disease, inflamm atory polyp, hyperplastic polyp, but ulcerative colitis, adenoma and colonic carcinoma occur quantitative and/or qualitative changes in the nomal pattern of mucus secretion. These changes are characterized by predominance of sialomucins, especially, an increase in blue staining in the PAT—KOH—PAS technique in that lesions with mode rate or severe dysplasia. It suggested that the hetergeneous mucin could be taken as a signal of abnormal cellular differentiation.

关 键 词：粘液 组织化学 大肠肿瘤 癌前状态 

分 类 号：R735.34[医药卫生—肿瘤]