酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用  

Enhancement of Immune Response in Gibel Carp (Carassius auratus gibelio) Inoculated with Aeromonas hydrophila Bacterin Using Yeast β-Glucan

作　　者：陈超然[1] 陈萱[2] 陈昌福[1] 姚娟[3] 梁运祥[2] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]华中农业大学农业微生物国家重点开放实验室,武汉430070 [3]湖北安琪酵母股份有限公司,宜昌443003

出　　处：《华中农业大学学报》2003年第4期380-384,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金资助项目 ( 3 0 2 710 3 2 )

摘　　要：在饲料中添加定量的酵母 β -葡聚糖 ,投喂经注射接种福尔马林灭活的嗜水气单胞菌 (Aeromonashydrophila)菌苗的异育银鲫 (Carassiusauratusgibelio) 2 8d后 ,通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性 (SGPT)、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率 (RPS) ,探讨了酵母 β -葡聚糖对受免异育银鲫免疫应答的增强作用。结果表明 ,投喂添加 2 0 0 .0mg/ (kg·d)酵母 β -葡聚糖的饲料 ,不仅可以提高受免异育银鲫对灭活嗜水气单胞菌的免疫应答水平 ,增强抵抗嗜水气单胞菌人工感染的能力 ,而且还具有一定的促进生长和改善肝功能的作用。Gibel carp (Carassius auratus gibelio) were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A. hydrophila. The results showed that adding yeast β-glucan with a ratio of 200.0 mg/(kg·d) into the diets can not only enhance the immune response and the resistance of gibel carp to A. hydrophila, but also improve their liver function and promote their growth to some degree.

关 键 词：酵母Β-葡聚糖 异育银鲫 免疫应答 增强作用 嗜水气单胞菌 饲料添加剂 细菌性败血症 

分 类 号：S943.117[农业科学—水产养殖] S942.5[农业科学—水产科学]