β-环糊精葡萄糖基转移酶高产菌株02-5-71的选育及发酵条件研究  

STUDY ON THE SCREENING AND FERMENTATION OF CYCLODEXTRIN GLUCANOTRANSFERASE HIGH-PRODUCTION STRAINS

作　　者：王雁萍[1] 王付转[1] 李宗伟[1] 吴健[1] 陈林海[1] 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《郑州工程学院学报》2003年第3期71-73,共3页

基　　金：河南省科技攻关项目(001160201)

摘　　要：应用氮离子注入对嗜碱芽孢杆菌进行诱变育种,获得了产生β-环糊精葡萄糖基转移酶是出发菌株2倍以上的高产菌株,酶活力可达6000U/mL以上.应用正交实验法筛选出最优发酵条件.The cyclodextrin glucanotransferaseproducing strain was treated with nitrogen ionbeam injection. A mutant was obtained and its enzyme productivity was above 6 000 U/ mL ,that was 2fold higher than that of parent strain. The best fermentative condition was selected using orthogonal test .

关 键 词：β-环糊精葡萄糖基转移酶 氮离子注入 嗜碱芽孢杆菌 诱变育种 生产菌株 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]