应用单克隆抗体免疫荧光技术检测马铃薯青枯病潜伏侵染的初步研究  

MONOCLONAL ANTIBODIES USED TO DETECT LATENT BACTERIAL WILT INFECTIONS BY AN INDIRECT FLUORESCENT ANTIBODY STAINING PROCEDURE IN POTATOES

作　　者：谢云陆[1] 高德香 何礼远[1] 王远程[2] 蔡少华[2] 

机构地区：[1]中国农科院植物保护研究所 [2]中国农科院生物技术中心

出　　处：《马铃薯杂志》1992年第1期2-6,共5页Chinese Potato Journal

摘　　要：应用单克隆抗体免疫荧光技术(MIF)检测了青枯病在马铃薯种薯上的潜伏侵染。测试结果表明,MIF检测青枯病茵的灵敏度为2×10~3～2×10~4细菌/毫升。787.5倍荧光显微镜,每视野平坶有5个以上典型荧光细菌,即可判别为带菌(阳性),而1～4个荧光细菌则视为可疑,需作生物测定。从种薯脐部取样,经组织匀浆后采用低、高速离心收集细菌体,可提高样品青枯菌的检出率。Theroutine method(Oil content:'remains method'; Protein content: Kajadal method)and the near-IR method for determination for coarse oil and protein contents were compared and analysed in this paper. The results indicated that: the coarse oil and protein contents ofsoybeans determined by routine method and that by the near-IR was notsignificantly different. Therefore, the near-IR could be substitute for the routine method, The colour of soybean seed coat had no effect on accuracy of determination by the near-IR.

关 键 词：马铃薯 青枯病 侵染 单克隆抗体 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]