癌相关基因在结肠癌中的表达及其甲基化调控  

Regulation of DNA methylation on the expression of tumor suppressor gene and oncogene in human colon cancer cell lines

作　　者：陆娟[1,2] 房静远[1,2] 陈萦晅[1,2] 朱红音 童菊芳[1,2] 杨丽 

机构地区：[1]上海第二医科大学附属仁济医院,上海200001 [2]上海市消化疾病研究所

出　　处：《肿瘤》2003年第4期290-293,共4页Tumor

基　　金：国家自然科学基金项目资助 (编号 :3 0 170 413 );高等学校全国优秀博士学位论文作者专项资助基金(编号 :199946)

摘　　要：目的　探讨癌相关基因APC、p16 INK4A、p2 1WAF1和c myc等在结肠癌中的表达及其受甲基化的调控。 方法　培养结肠癌细胞SW 1116、Colo 32 0、HT2 9,分别用不同浓度的 5 aza dC干预细胞。提取细胞的RNA ,用RT PCR的方法检测p16 INK4A、p2 1WAF1、APC和c myc等多种基因的表达情况 ;同时以流式细胞仪分析SW1116和Colo 32 0的细胞周期。结果　 (1)三种细胞在干预前有较弱的 p16 INK4A和APC的表达 ,而在用 5 aza dC干预后表达增强 ,且在不同的结肠癌细胞株 5 aza dC作用发挥最佳的时间与浓度不同。 (2 ) p2 1WAF1在干预前后均不表达 ,c myc在干预前后表达无明显变化。 结论　三株人结肠癌细胞中 ,5 aza dC均可诱导 p16 INK4A和APC的表达 ,但对 p2 1WAF1和c myc无明显影响。Objective To investigate the effects of DNA methylation on tumor suppressor gene and oncogene in human colon cancer cells. Methods Three colon cancer cell lines(HT29, SW1116 and Colo 320) were treated with the DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine (5-aza-dC). The expression of p16 INK4A , p21 WAF1 , APC and c-myc was assayed by RT-PCR. Cell cycle analysis by flow cytometry was performed. Results The expression of p16 INK4A and APC were slightly detected in all three colon cancer cells. However, the expressions of them were elevated to different extent after exposing to 5-aza-dC with different concentration and time for SW1116 and Colo 320 cells. p21 WAF1 didn't expressed before and after 5-aza-dC treatment. The expression of c-myc was not significant different in SW116 and Colo-320 cells treated by 5-aza-dC. Conclusion The expression of p16 INK4A and APC genes was regulated by DNA methylation in three human colon cancer cells.

关 键 词：结肠癌 APC基因 P16^INK4A基因 P21^WAF1基因 c-myc基因 DNA甲基化 5-氮脱氧胞苷 

分 类 号：R735.35[医药卫生—肿瘤]