肝脏疾病患者血浆未γ-羧基化蛋白C水平及其临床意义  

Levels of Plasma Des-γ-Carboxy Protein C in Patients with Liver Diseases and its Clinical Significance

作　　者：何晓凡[1] 文志斌[1] 熊石龙[1] 汉建忠[1] 刘敏涓[2] 周立红[2] 李群[3] 贺石林[1] 

机构地区：[1]中南大学湘雅医学院生理学系止血生理实验室,中国湖南长沙410078 [2]广州医学院第二附属医院 [3]中南大学湘雅医院血液科

出　　处：《实用预防医学》2003年第4期442-444,共3页Practical Preventive Medicine

基　　金：国家教育部优秀青年教师基金 (教人司 2 0 0 1 :39号 )资助;国家自然科学基金委资助项目 :C3960 0 1 97

摘　　要：目的　观察肝脏疾病患者血浆未 γ-羧基化蛋白 C(DCPC)水平及蛋白 C活性 (PC:C)、抗原 (PC:Ag)含量的变化 ,探讨血浆未 γ-羧基化蛋白 C和蛋白 C活性、抗原的检测对肝脏疾病的临床意义。　方法　PC:C测定采用发色底物法 ;PC:Ag采用 EL ISA法 ;DCPC亦采用 EL ISA法检测。　结果　与正常对照组相比 :1肝癌 (原发性 )、肝硬化患者血浆 PC:C和 PC:Ag均明显降低 (P<0 .0 5 ,) ;急性黄疸型乙型肝炎患者血浆 PC:Ag含量降低 (P<0 .0 1) ,而 PC:C的差异无显著意义 (P>0 .0 5 )。 2 DCPC水平在肝癌组明显升高 (P<0 .0 5 ) ,大于总 PC:Ag的 5 0 % ;而急性黄疸型乙型肝炎组 DCPC差异无显著意义 (P>0 .0 5 )。　结论　 1)肝癌、肝硬化患者血浆 PC:C、PC:Ag水平均降低。 2 )由于高水平的DCPC。Objective To study plasma des-γ-carboxy protein C (DCPC),protein C activity(PC:C)and protein C antigen(PC:Ag) levels in patients with liver diseases, and explore its clinical significance. Methods The plasma PC:C was detected by chromogenic assay and PC:Ag was detected by ELISA. DCPC was also detected by ELISA. Result Compared with the control,:① The levels of PC:C and PC:Ag in patients with hepatocellular carcinoma (HCC) and liver cirrhosis were lower (p<0.05). PC:Ag in acute viral hepatitis (AVH) also was lower(P<0.01), but PC:C was closed to the control (p>0.05). ② The levels of DCPC in patients with HCC were remarkably higher (p<0.05) and its average was up to 50% of total PC:Ag. But those of DCPC in patients with AVH were not significantly different to the control. Conclusion (1).PC:C and PC:Ag in patients with liver diseases(except PC:C in AVH) were lower. (2). DCPC may be used as a complementary marker in the diagnosis of HCC because of its high level.

关 键 词：肝脏疾病 蛋白C 未γ-羧基化蛋白C(DCPC) 

分 类 号：R575[医药卫生—消化系统]