诱变筛选荧光假单胞菌M18高产吩嗪-1-羧酸(PCA)菌株及发酵条件研究  

Mutation of Pseudomonas fluorescens sp. M18 producing phenazine carboxylic acid and its fermentation conditions

作　　者：耿海峰[1] 陈峰[1] 许煜泉[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《工业微生物》2003年第3期1-5,共5页Industrial Microbiology

基　　金：国家"十五"科技攻关项目 ( 2 0 0 1BA3 0 8A0 2 14 );上海市科技兴农攻关项目 ( 2 0 0 15 11)资助

摘　　要：研究了亚硝基胍 (NTG)的诱变剂量、处理时间以及氯化钠浓度对野生型荧光假单胞菌M 18存活率的影响 ,NTG的诱变剂量 2 5～ 2 0 0mg/L ,处理时间 10～ 30min范围内 ,随着诱变剂剂量增加和时间的延长 ,M 18的存活率不断下降。NTG的作用剂量 2 5mg/L ,处理时间 10min时 ,细菌存活率为 5 5 %左右。利用细菌存活率为 5 5 %时的诱变条件 ,经生物测定 ,初筛获得 2 0株抑菌效果明显的诱变株。经摇瓶发酵复筛 ,从这 2 0株诱变株中 ,获得PCA高产诱变株M 18N0 7。并对此菌株发酵条件进行调整 ,较理想的培养条件为 :蛋白胨 18g/L ,葡萄糖 16 g/L ,氯化钠 1g/L ,硝酸钾10 g/L。小瓶培养时间 17h ,大瓶 5 %接种量 ,2 8℃培养 5 4h。The offects of the concentration and working time of NTG ,as well as the concentration of NaCl on the living rate of Pseudomonas fluorescens sp. M18 was investigated. When the concentration of NTG was 25～200mg/L and working time was 10～30min, the living rate of M18 was decreased with addition of the concentration and time. If the concentration of NTG was 25mg/L and the working time was 10min,the living rate of M18 was 55%.Under these conditions , 20 potential stains with significant biocontrol effects were obtained,especially the strain Pseudomonas fluorescens sp. M18N07 from above potential strains with high yield of PCA. The fermentation conditions for the mutant strain was then optimized. The optimal medium for PCA production was:protein 18g/L,glucose 16g/L,NaCl 1g/L,KNO 3 10g/L, 50mL(250mL) flask for 17 hours,5%(v/v) inoculation, 150mL(500mL) flask for 54 hours,28℃.

关 键 词：荧光假单胞菌 吩嗪-1-羧酸 PCA 发酵条件 诱变 

分 类 号：TQ920[轻工技术与工程—发酵工程]