玉米蛋白粉中黄体素和玉米黄素的分离与定性  

Isolation and Identification of Lutein and Zeaxanthin from Corn Gluten by Thin-Layer Chromatography

作　　者：卢艳杰[1] 陈正行[1] 姚惠源[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第5期11-14,24,共5页Journal of Wuxi University of Light Industry

基　　金：国家863专项课题项目(2001AA248011)资助课题.

摘　　要：利用溶剂萃取、薄层层析对玉米蛋白粉中黄体素和玉米黄素进行了提取和分离.通过薄层层析的相对迁移率、与AgNO3的显色反应、紫外 可见吸收光谱、高效液相色谱保留时间及液质联用所得到的相对分子质量信息,对分离所得黄体素和玉米黄素进行了定性研究,为玉米蛋白粉中黄体素和玉米黄素的进一步研究和开发利用奠定了基础.Solventextraction and thinlayer chromatography were used to extract and separate lutein and zeaxanthin from corn gluten. Their structures were identified by comparing the Rf value with the standard, the color reaction with AgNO3, the UVspectrum, the retention time of RPHPLC, and the spectrum of LC/MS. The results provided the basic information for the further research.

关 键 词：玉米蛋白粉 黄体素 玉米黄素 分离 薄层层析 提取 

分 类 号：TS212[轻工技术与工程—粮食、油脂及植物蛋白工程]