微波辅助萃取气相色谱-质谱联用测定蔬菜中的扑草净  

Determination of prometryne in vegetable samples by GC-MS using microwave-assisted extraction

作　　者：杨云[1] 栾伟[1] 李攻科[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275

出　　处：《分析试验室》2003年第4期75-78,共4页Chinese Journal of Analysis Laboratory

基　　金：国家重点基础研究发展规划项目(批准号G2000026302);教育部留学回国人员科研启动基金项目

摘　　要：研究了微波辅助萃取气相色谱 质谱联用测定植物样品中扑草净的方法,比较了几种不同溶剂的微波萃取效率,从而选取二氯甲烷为萃取溶剂,并采用三因素三水平的正交设计试验对溶剂体积、微波辐射时间、微波功率进行了优化。在优化的实验条件下分析了合成菠菜样品,对0.2μg/g和0.02μg/g的合成菠菜样品,回收率分别为99.5%和92.5%,相对标准偏差分别为5.0%和11%,方法的线性范围为1.0～400ng/g,检出限为0.22ng/g。方法适合于分析植物样品中的扑草净。A microwaveassisted extraction (MAE) method for the determination of prometryne in vegetable samples by GCMS was developed. Different extraction solvents were compared for the extraction of prometryne in vegetable samples. Dichloromethane was the selected as the extraction solvent because it yielded good recovery, and was very convenient for the following procedure. The MAE conditions, such as the extraction solvent volume, microwave radiation time, and power output, were optimized by a threelevel orthogonal array design. The synthetic vegetable samples were determined under the optimum conditions, the recoveries were 92.5%～99.5% with RSD of 11%～5.0% (n=3). The results agreed quite well with those obtained by a conventional mechanically shaking extraction. The linear range of the method was from 1.0 to 400 ng/g with the detection limit of 0.22 ng/g.

关 键 词：微波辅助萃取 气相色谱-质谱联用 测定 蔬菜 扑草净 除草剂 药物残留 

分 类 号：R155.54[医药卫生—营养与食品卫生学] S481.8[医药卫生—公共卫生与预防医学]