气相色谱-串联质谱法检测水产品中氯霉素残留量  

Determination of chloramphenicol residues in aquatic products by gas chromatography-tandem mass spectrometry

作　　者：宋宏娟 

机构地区：[1]新疆维吾尔自治区水环境监测中心,乌鲁木齐830000

出　　处：《食品安全质量检测学报》2015年第6期2373-2378,共6页Journal of Food Safety and Quality

摘　　要：目的建立检测分析水产品中氯霉素残留量的气相色谱-串联质谱联用（GC-MS/MS）的方法。方法样品采用乙酸乙酯提取、经过C₁₈固相萃取柱净化,用硅烷化试剂衍生化,采用GC-MS/MS分析时,氯霉素在15min内流出。结果对样品添加两种浓度的加标质控,回收率在80%以上,相对标准偏差（RSD）在6.2%以内。在0.5¹0 mg/L质量浓度范围内呈线性关系,相关系数大于0.99。结论该方法可用于氯霉素残留的准确定量分析,灵敏度、精密度和准确度均能满足渔药残留检测分析要求。ABSTRACT:Objective To establish a determination method of chloramphenicol (CAP) in aquatic products by gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The samples were extracted by ethyl acetate, cleaned up by C18 SPE column, and derived with silanization reagent, then the extracts were determined by MS/MS detector based on SCAN and MRM mode. Results CAP was well separated within 15 min. This method showed that the spiked average recoveries above 80%, the RSD ranged within 6.2%, and the calibration of those pesticides were linearity within the range of 0.5~10 mg/L (r2>0.99). Conclusion The method is accurate and sensitive, it is suitable for analysis of CAP residues in aquatic products.

关 键 词：氯霉素 气相色谱-串联质谱法 药物残留 水产品 

分 类 号：O657.63[理学—分析化学] TS254.7[理学—化学]