抑菌剂在异养小球藻发酵过程中的应用  

The application of antibacterial agent in the cultivation of heterotrophic Chlorella

作　　者：毕生雷 张成明[2,3] 金洪波[1] 郑世文[1] 刘钺[1] 杜风光[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [2]清华大学核能与新能源技术研究院,北京100084 [3]北京市生物燃料工程技术研究中心,北京100084

出　　处：《食品与发酵工业》2015年第3期70-74,共5页Food and Fermentation Industries

基　　金："十二五"农村领域国家科技计划课题研究任务合约"非粮生物质和CO2生产高产油微藻产业化示范"的支持(No.2011BAD14B05)

摘　　要：通过抑菌圈试验、摇瓶发酵试验和50 L发酵试验,研究了安菌素、克菌灵、青霉素3种抑菌剂在小球藻培养过程中的应用,考察了抑菌剂添加对藻细胞干重和葡萄糖浓度的影响。结论:在小球藻发酵染菌4 d后,加入100 mg/L克菌灵可以有效抑制杂菌,并且不会对小球藻发酵造成影响。相同发酵周期内,小球藻染菌后抑菌发酵与正常发酵相比,藻细胞干重质量浓度达到75.2 g/L,平均干重增加速率为6.26 g/(L·d),与正常发酵相比,干重质量浓度与平均干重增加速率降低14.93%,能够成功地挽救染菌的发酵过程。而加入100 mg/L安菌素和青霉素均对小球藻的正常生长产生了抑制作用。Through the bacteriostatic ring experiment,shake flask test,and 50 L fermentation experiment,the applications of three novel antimicrobial agents such as Anjunsu,Kejunling,Penicillin in Chlorella cultivation process were studied. The changes of glucose concentration and dry weight after adding antibacterial agent in the process of fermentation were compared. In conclusion,addition of 100 mg / L Kejunling after infection for 4 d could effectively inhibit the bacteria and would not affect the normal Chlorella fermentation process. At the same fermentation time,the dry weight could reach 75. 2 g / L with the average daily dry weight increased rate of 6. 26 g /( L ·d),which were14. 93% lower than that of the normal fermentation process,so the infected fermentation process could be successfully saved by Kejunling. While additions of 100 mg / L grams of Anjunsu and Penicillin both inhibited the growth of Chlorella.

关 键 词：抑菌剂 小球藻 安菌素 克菌灵 青霉素 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]