Caco-2细胞模型构建及抗高血压肽VPP和IPP小肠吸收机制研究  

Establishment of Caco-2 Cell Model and Intestinal Absorption Mechanism of the Antihypertensive Peptides Val-Pro-Pro and Ile-Pro-Pro

作　　者：祝倩[1] 郭宇星[1] 潘道东[1,2] 曾小群[2] 孙杨赢[2] 周慧敏[1] 

机构地区：[1]南京师范大学金陵女子学院,江苏南京210097 [2]宁波大学海洋学院,浙江宁波315211

出　　处：《食品科学》2014年第15期226-231,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31101314);江苏省自然科学基金项目(BK2011787);江苏省高校自然科学研究项目(13KJB550013);浙江省自然科学基金项目(LQ12C20003);宁波市科技局自然科学基金项目(2012A610145)

摘　　要：Caco-2细胞模型为小肠内皮细胞转运模型,本实验构建Caco-2细胞模型并研究抗高血压肽Val-Pro-Pro(VPP)和Ile-Pro-Pro(IPP)的小肠吸收机制。从细胞形态、电阻值和荧光素钠透过率等方面验证了Caco-2细胞模型;通过转运时间、肽浓度、吸收抑制剂和促进剂比较了VPP和IPP的小肠转运途径及外排机制。结果表明:构建的Caco-2细胞模型可用于VPP和IPP的模拟小肠吸收机制研究;VPP和IPP的小肠转运途径是旁路转运,VPP和IPP都存在外排泵的作用,IPP外排作用没有VPP大,所以生物利用度高于VPP。The Caco-2 cell model, an intestinal endothelial model for transport study, was established to study the absorption mechanism of the antihypertensive peptides, Val-Pro-Pro(VPP) and Ile-Pro-Pro(IPP). The Caco-2 cell model was evaluated by morphological features, transepithelial electrical resistance and the transmittance of fluorescein sodium. We comparatively investigated the effects of transport time, peptide concentration, enhancers and inhibitors on the transport pathways and efflux mechanisms of VPP and IPP. The results showed the established Caco-2 cell model could be used to study the intestinal absorption of VPP and IPP. Paracellular diffusion was suggested to be the main mechanism for the absorption of the two antihypertensive peptides. The transports of both peptides were influenced by the efflux pump. The efflux affected VPP more than IPP. Thus, IPP showed higher bioavailability.

关 键 词：抗高血压肽 Val-Pro-Pro Ile-Pro-Pro CACO-2细胞 转运 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]