毛细管电泳-电化学发光分离检测辣椒粉中罗丹明B  

Separation and Determination of Rhodamine B in Chili Power by Capillary Electrophoresis with Electrochemiluminescence Detection

作　　者：屈忠凯[1] 邓光辉[1] 王士伟[1] 王辉[1] 方梦霞[1] 韦福赛 刘清鸿 

机构地区：[1]广西民族大学化学化工学院广西高校化学与生物转化过程新技术重点实验室,广西南宁530006

出　　处：《食品科学》2015年第4期217-220,共4页Food Science

基　　金：广西高校人才小高地建设创新团队资助计划项目(桂教人[2011]47号12)

摘　　要：建立一种以三联吡啶钌(Ru(bpy)32+)为发光体系的毛细管电泳-电化学发光检测系统,并将其应用于分离和测定辣椒粉中的罗丹明B含量。考察检测电压、Ru(bpy)32+溶液浓度、磷酸盐缓冲液的p H值和浓度、分离电压、进样电压与进样时间等因素对分离检测的影响。结果表明:在检测电压1.14 V、Ru(bpy)32+溶液浓度5 mmol/L、磷酸盐缓冲溶液浓度20 mmol/L(p H 8.0)、进样时间10 s、进样电压10 k V、分离电压15 k V条件下,罗丹明B在6 min中得到分离。方法的线性范围为5×10-7~5×10-5 mol/L,检出限为1×10-7 mol/L(RSN=3)。对1.0×10-5 mol/L的罗丹明B标准溶液连续测定5次,迁移时间和峰面积的相对标准偏差分别为3.2%和1.5%。该方法可成功应用于辣椒粉中罗丹明B含量的测定。A sensitive and simple method based on capillary electrophoresis(CE) with electrochemiluminescence(ECL) detection has been developed for the separation and determination of rhodamine B in chili power. The effects of detection potential, the concentration of Ru(bpy)32+, the acidity and concentration of the running buffer, separation voltage and injection time were investigated to acquire the optimum conditions. The detection electrode was a 300 μm diameter platinum microelectrode at a detection potential of 1.14 V(versus Ag/Ag Cl). Rhodamine B could be well separated within 6 min in a 50 cm length fused-silica capillary at a separation voltage of 15 k V in a 20 mmol/L PBS buffer(p H 8.0). The response range of rhodamine B was from 5 × 10-7 to 5 × 10-5 mol/L with a detection limit of 1 × 10-7 mol/L(RSN = 3). The proposed method demonstrated long-term stability and reproducibility with relative standard deviations(RSD) of less than 3.5% for both migration time and electrophoretic peak area(n = 5). This method can be successfully applied to the analysis of rhodamine B in chili power with satisfactory assay results.

关 键 词：毛细管电泳 电化学发光 辣椒粉 罗丹明B 

分 类 号：O657[理学—分析化学] TS264[理学—化学]