土壤类型对樱桃番茄VC含量及其相关酶活性的影响  

Effect of Soil Type on Vitamin C Content and Activities of Related Enzymes in Cherry Tomato

作　　者：包天莉 梁银丽[1,2] 李文平[3] 穆兰[2] 高德凯 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]中国科学院水利部水土保持研究所,陕西杨凌712100 [3]西北农林科技大学林学院,陕西杨凌712100

出　　处：《食品科学》2015年第5期24-28,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2014BAD14B006;2011BAD31B05)

摘　　要：以樱桃番茄(Lycopersicon esculentum var.cerasiforme)为材料,研究温室内外及塿土黄绵土条件下,樱桃番茄果实VC含量和抗坏血酸氧化酶(ascorbic acid oxidase,AAO)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、多酚氧化酶(polyphenol oxidase,PPO)活性的变化。结果表明:从结果初期到结果末期,VC含量呈先升后降的趋势,AAO活性变化先降后升,APX活性呈上升趋势,PPO活性则随着生育期的进行不断下降。同一环境条件下,塿土樱桃番茄果实VC含量高于黄绵土;同一土类情况下,露天处理优于温室处理。整个生育期内,塿土露天处理樱桃番茄果实VC含量显著高于其他处理,不同处理间差异显著(P<0.05)。温室黄绵土处理樱桃番茄果实抗坏血酸氧化酶活性最高,露天塿土最低。结果初期APX活性大小顺序为露天塿土>温室塿土>温室黄绵土>露天黄绵土,结果盛期和结果末期均为露天塿土>露天黄绵土>温室塿土>温室黄绵土。而PPO活性在露天黄绵土处理下最高,露天塿土处理次之。果实鲜质量为温室塿土最高。相关分析表明,VC含量与AAO和PPO活性呈负相关。由VC含量与不同酶类之间的关系及各处理对其影响的结果综合分析:在保持相对高VC含量,低AAO、APX和PPO活性的情况下,露天塿土处理最佳。The cherry tomato(Lycopersicon esculentum var.cerasiforme) was used to investigate vitamin C(VC) content,ascorbic acid oxidase(AAO),ascorbate peroxidase(APX) and polyphenol oxidase(PPO) activities under greenhouse and outdoor condition with Lou soil or loessial soil during the fruiting period.The results showed that from early to later fruiting stages,the VC content increased fi rstly then decreased,whereas the AAO activity exhibited the opposite trend.Meanwhile,APX activity showed an increasing trend while PPO activity presented a decreasing trend.The VC content was higher in cherry tomatoes from Lou soil than those from loessial soil under the same culti vation condition and it was better in outdoor than in greenhouse with the same type of soil.The vitamin C content was signifi cantly higher in outdoor Lou soil cultivation than other treatments during the growth seasons.A significant difference between o utdoor Lou soil culture and other treatments was observed(P < 0.05).However,AAO activity was the highest in greenhouse loessial soil treatment and the lowest in outdoor Lou soil treatment.The ascorbate peroxidase activity followed the order of outdoor Lou soil > g reenhouse Lou soil > greenhouse loessial soil > outdoor loessial soil during the early fruiting stage,while followed the order outdoor Lou soil > outdoor loessial soil > greenhouse Lou soil > greenhouse loessial soil during the full fruiting stage and later fruiting stage.PPO activity was the highest in outdoor loessial soil treatment and outdoor Lou soil treatment took second place.Correlation analysis indicated that the activities of AAO and PPO were negatively correlated with ascorbic acid content.Therefore,outdoor Lou soil was optimum treatment for preserving high ascorbic acid content and low AAO,APX and PPO activities.

关 键 词：樱桃番茄 抗坏血酸 抗坏血酸氧化酶 抗坏血酸过氧化物酶 多酚氧化酶 

分 类 号：S641.2[农业科学—蔬菜学]