冬虫夏草与蛹虫草融合株的原生质体诱变育种  

Ultraviolet-Induced Mutagenesis of Cordyceps sinensis and Cordyceps militaris Protoplast Fusant

作　　者：曲鸿雁[1] 郭成金[1] 

机构地区：[1]天津师范大学生命科学学院天津市动植物抗性重点实验室,天津300387

出　　处：《食品科学》2015年第5期120-125,共6页Food Science

基　　金：国家星火计划项目(2012GA610002)

摘　　要：以冬虫夏草与蛹虫草融合株为研究材料,对其原生质体进行紫外诱变,筛选优质高产的新型菌株。得到了高产菌株Y-90,虫草酸产量是出发菌株的1.80倍,高产菌株YP-42,虫草素产量是出发菌株的1.33倍。采用随机扩增多态性DNA(random amplifi ed polymorphic DNA,RAPD)分子标记技术和核糖体r DNA内部转录间隔区(internal transcribed spacer,ITS)序列分析技术对突变株的遗传变异情况进行分析。结果表明突变株的ITS序列并没有发生变化。RAPD结果显示突变株的扩增片段与亲本菌株不同,说明发生了基因突变,其高产性状是可遗传的性状,可以将其用于进一步的育种研究。Protoplast fusants between Cordyceps sinensis and Cordyceps militaris were mutagenized by ultraviolet(UV) irradiation for improved production of cordycepic acid and cordycepin in Cordyceps mycelia.Two mutant strains(Y-90 and YP-42) were selected showing a 1.80-and 1.33-fold increase in the production of cordycepic acid and cordycepin when compared with the original funsant strain,respectively.Random amplifi ed polymorphic DNA(RAPD) markers and ribosomal DNA internal transcribed spacer(r DNA-ITS) sequence analysis were used to analyze the genetic variability.The ITS sequence comparison revealed no genetic change after mutagenesis.RAPD analysis showed that the amplified fragments from the two mutants were different from the parent strain,and the acquired characters of the mutants were caused by gene mutation.

关 键 词：冬虫夏草 蛹虫草 紫外诱变 虫草酸 虫草素 子实体 随机扩增多态性DNA RDNA-ITS 

分 类 号：S567.35[农业科学—中草药栽培]