泰山蛹虫草多糖对免疫抑制小鼠肠道菌群及分泌型免疫球蛋白A的影响  

Effect of Cordyceps taishanensis Polysaccharide on Intestinal Microfl ora and Secretory Immunoglobulin A in Immunosuppressive Mice

作　　者：张圣方 赵龙玉[1] 赵凤春[1] 王雪[1] 杨正友[1] 

机构地区：[1]山东农业大学生命科学学院山东省农业微生物重点实验室,山东泰安271018

出　　处：《食品科学》2015年第5期148-152,共5页Food Science

基　　金：国家自然科学基金面上项目(30972050;31271873);山东省自然科学基金项目(ZR2010CM015)

摘　　要：目的:研究泰山蛹虫草多糖对免疫抑制小鼠肠道菌群和肠黏膜分泌型免疫球蛋白A(secretory immunoglobulin A,s Ig A)的影响。方法:将60只雄性昆明小鼠随机平均分为5组,空白对照组和环磷酰胺(cyclophosphamide,CY)模型组的小鼠灌胃蒸馏水,3个蛹虫草多糖组分别以100、200、300 mg/(kg?d)(以体质量计,下同)剂量的泰山蛹虫草多糖灌胃小鼠19 d。第20天,空白对照组小鼠腹腔注射生理盐水,其余4组小鼠均腹腔注射100 mg/kg的CY。观察蛹虫草多糖对小鼠肠道菌群和肠黏膜s Ig A水平的影响。结果:与CY模型组比较,3个蛹虫草多糖组小鼠肠道的双歧杆菌、乳酸杆菌数量均极显著增加(P<0.01),而大肠杆菌、肠球菌数量均显著或极显著降低(P<0.05或P<0.01);3个蛹虫草多糖组小鼠的肠道s Ig A含量均极显著升高(P<0.01)。结论:泰山蛹虫草多糖对CY所致的免疫抑制小鼠肠道菌群紊乱有拮抗作用;泰山蛹虫草多糖也能促进肠黏膜s Ig A的分泌,在一定程度上拮抗CY所致的肠黏膜免疫抑制。This study was conducted aiming to explore the effect of Cordyceps taishanensis polysaccharide on the intestinal microflora and intestinal mucosal secretory immunoglobu lin A(s Ig A) in immunosuppressive mice.Sixty Kunming male mice were randomly divided into five groups including control group,cyclophosphamide(CY) model group and C.taishanensis polysaccharide treatment groups.The mice of both control and CY model groups were administrated with distilled water,and those in three treatment groups were administrated with C.taishanensis polysaccharide at doses of 100,200 and 300 mg/(kg·d),respectively,by gavage for 19 successive days.On the 20 th day,the mice from control group were intraperitoneally injected with normal saline,and those from four other groups were intraperitoneally injected with CY at a dose of 100 mg/(kg·d),and the effect of C.taishanensis polysaccharide on the intestinal microfl ora and intestinal mucosal s Ig A were analyzed.Compared with the CY model group,the numbers of Bifi dobacterium and Lactobacillus were signifi cantly increased(P < 0.01),the numbers of Escherichia coli and Enterococcus were signifi cantly decreased(P < 0.05 or P < 0.01) and the content of intestinal mucosal s Ig A was signifi cantly increased(P < 0.01) in three treatment groups.These results indicated that C.taishanensis polysaccharide could regulate the disturbance of th e intestinal microflora in immunosuppressive mice induced by CY and could also antagonize CY-induced damage of intestinal mucosal immunity to a certain extent by promoting the secretion of s IgA.

关 键 词：泰山蛹虫草 多糖 环磷酰胺 免疫抑制小鼠 肠道菌群 分泌型免疫球蛋白A 

分 类 号：R151[医药卫生—营养与食品卫生学]