邻苯二甲酸二(2-乙基己基)酯在青春期小鼠体内的代谢动力学研究  

Pharmacokinetics of Di-(2-ethylhexyl)phthalate in Pubertal Mice

作　　者：张丹[1] 叶逵[1] 王家家[1] 徐海[1] 宋星星[1] 金忠秀[1] 盛杰[1] 李李[1] 刘开永[1] 

机构地区：[1]安徽医科大学公共卫生学院,安徽合肥230601

出　　处：《食品科学》2015年第5期185-188,共4页Food Science

基　　金：安徽省高校省级自然科学研究项目(KJ2012Z168);安徽医科大学中青年学术骨干资助基金项目(2012051);安徽医科大学博士科研基金项目(XJ201112)

摘　　要：研究邻苯二甲酸二(2-乙基己基)酯(di-(2-ethylhexyl)phthalate,DEHP)在青春期小鼠体内的代谢动力学,以300 mg/kg DEHP灌胃青春期小鼠,血清样品经固相萃取(solid phase extraction,SPE)提取和净化,气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)法测定不同时间点的血药质量浓度,以3p97软件拟合其动力学参数。DEHP在青春期小鼠体内的主要药物代谢动力学参数:药峰质量浓度ρmax为(0.47 6±0.028)μg/m L,达峰时间Tpeak为(2.539±0.424)h,药-时曲线下面积AUC(0-t)为(32.209±11.220)h·μg/m L,分布半衰期t1/2α为(1.886±0.480)h,消除半衰期t1/2β为(36.473±8.324)h。本方法简便快捷,其灵敏度和准确度均可满足青春期小鼠体内DEHP代谢动力学研究。DEHP在青春期小鼠体内药物代谢动力学过程符合一级吸收二室开放模型,其分布较快,代谢较慢。A pharmacokinetic study of di-(2-ethylhexyl)phthalate(DEHP) was carried out in pubertal mice.The serum of pubertal mice was collected after oral administration of DEHP at a dose of 300 mg/kg,and DEHP was extracted from the serum samples and purifi ed by solid phase extraction(SPE).The DEHP concentrations in serum at different time points were assayed by gas chromatography-mass spectrometry(GC-MS) and the pharmacokinetic parameters were calculated by 3p97 software.The major pharmacokin etic parameters of DEHP in pubertal mice were obtained as follows:ρmax(0.476 ±0.028) μg/m L,Tpeak(2.539 ± 0.424) h,AUC(0-t)(32.209 ± 11.220) h·μg/m L,t1/2α(1.886 ± 0.480) h,and t1/2β(36.473 ±8.324) h.The method presented is sensitive,accurate and fast,and can meet the requirements for the pharmacokinetic study of DEHP in pubertal mice.The pharmacokinetics of DEHP in pubertal mice was found to be fi tted to a two-compartment open model with fi rst order absorption and DEHP reveals the quick distribution and slow elimination.

关 键 词：药物代谢动力学 气相色谱-质谱联用 固相萃取 邻苯二甲酸二(2-乙基己基)酯 

分 类 号：R965[医药卫生—药理学]