真空冷冻干燥和保存兔角膜内皮细胞活性的实验研究  

Viability of Endothelium Cells of Rabbital Cornea With Vacuum Freeze-Drying and Storing

作　　者：陈晓隆[1] 杨宏伟[1] 刘哲丽[2] 徐成海[3] 王德喜[3] 

机构地区：[1]中国医科大学附属第二医院眼科,辽宁沈阳110004 [2]中国医科大学附属第一医院眼科 [3]东北大学机械工程与自动化学院

出　　处：《中国医科大学学报》2003年第5期414-416,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目 (5 98760 0 8)

摘　　要：目的 :观察真空冷冻干燥和保存的兔角膜的内皮细胞活性。方法 :真空冷冻干燥和保存兔角膜后 ,检测内皮细胞密度 ,存活率。结果 :真空冷冻干燥和保存的兔角膜复水后 ,角膜内皮细胞密度为 (2 339± 2 0 5 .73)个 /mm2 ,存活率 78.5 7%。结论Objective: Our aim was to observe the viability of endothelium cells on rabbital cornea dried and stored with vacuum freezing. Methods:Density of cornea endothelium cells and endothelium survival rate (ESR) were determined. Results:After dried and stored with vacuum freezing,the corneas were postwatered. Density of cornea endothelium cells was(2 339±205.73) cells/mm 2 and endothelium survival rate (ESR) was 78.57%. Conclusion:Postwatered rabbital corneas dried and stored with vacuum freezing could remain viability of endothelium cells.

关 键 词：真空冷冻 角膜 内皮细胞活性 

分 类 号：R779.65[医药卫生—眼科]