二氧化硫和铅联合染毒对小鼠遗传物质的损伤  

DNA damage in mice from sulfur dioxide and lead combined-poisoning.

作　　者：武冬梅[1] 孟紫强[1] 

机构地区：[1]山西大学环境医学与毒理学研究所,山西太原030006

出　　处：《中国环境科学》2003年第5期526-530,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(30070647)

摘　　要：运用单细胞凝胶电泳技术研究了SO2和醋酸铅亚急性联合染毒对雄性小鼠脑、肺、脾、肾细胞DNA的损伤.结果表明,在42mg/m3 SO2和0.2%醋酸铅单独及联合作用下,小鼠脑、肺、肾、脾细胞DNA迁移距离均比对照组显著增加,而且铅可以加剧SO2对脑、肾、脾细胞核DNA的损伤程度;SO2对肺细胞核DNA的损伤程度要比铅的损伤大,小鼠肺细胞核DNA迁移距离在SO2和醋酸铅联合作用组与醋酸铅单独作用组间有极显著性差异(P<0.01),而与SO2单独作用组间没有显著性差异.SO2和醋酸铅均可引起小鼠组织细胞的DNA损伤,它们对脑、肺、脾、肾的损伤程度不同,在一定条件下存在明显的协同效应,可以增强彼此的毒性作用.这意味着SO2和铅均有引起哺乳动物细胞基因突变的潜在危险,大气SO2污染与铅的污染可能加剧某些疾病的发生与发展.The DNA damage in the brain, lung, spleen and kidney cells of male mice from sulfur dioxide (SO2) and lead acetate sub-acute combined-poisoning was studied with the single cell microgel electrophoresis technique. The migratingdistances of DNA of brain, lung, spleen and kidney cells of mice increased significantly compared to the control group under conditions of single and combined poisoning of SO2 (42mg/m3) and lead acetate (0.2%), and lead could strengthen DNA damage degree by SO2 in nuclear DNA of brain, kidney, spleen cells. Damaging degree of SO2 on nuclear DNA oflung cell of mice was more severe than that of lead. The migrating distance of nuclear DNA of lung of mice in combined action group of SO2 and lead acetate had marked difference to that of lead acetate single action group (P<0.01); but no marked difference to SO2 single action group. It showed that both SO2 and lead acetate could induce DNA damage in mice cells; and their damaging degrees were different in brain, lung, spleen, kidney, and under certain conditions, there existed significant synergistic joint action, thus could enhance mutual toxicity. These suggested that both SO2 and lead could cause a potential risk of gene mutation of the cells of mammalian animals; air born SO2 and lead pollution could enhance the happening and developing of some diseases.

关 键 词：二氧化硫 醋酸铅 DNA损伤 小鼠 彗星实验 

分 类 号：X174[环境科学与工程—环境科学] R361[医药卫生—病理学]