大豆皂苷对α-葡萄糖苷酶抑制作用的研究  

Study on the Inhibition of alpha-Glucosidase by Soyasaponins

作　　者：全吉淑[1] 尹学哲[2] 金明[1] 沈明化[1] 

机构地区：[1]延边大学医学院,延吉133000 [2]延边大学医学院附属医院,延吉133000

出　　处：《中药材》2003年第9期654-656,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目 ( 3 9960 0 73 )

摘　　要：目的 :研究大豆皂苷对α 葡萄糖苷酶 (EC3 2 1 2 0 )的抑制作用。方法 :以C18反相柱层析和高效液相色谱法从大豆胚轴分离大豆皂苷 ,比色法测定大豆皂苷单体对α 葡萄糖苷酶的抑制活性。结果 :大豆皂苷具有很强的α 葡萄糖苷酶抑制作用 ,且表现为非竞争性抑制作用。其中B类、E类和DDMP皂苷的抑制作用为最强 ,其半抑制浓度 (IC50 )值为 10～ 4 0 μmol/L ;A类皂苷的抑制作用则较弱。结论 :大豆皂苷是α 葡萄糖苷酶抑制剂 ,经常食用可能有助于延缓糖尿病患者餐后血糖的持续升高。Objective:To study the inhibitory effects of soyasaponins on α-glucosidase (EC3.2.1.20). Methods:Soyasaponins were isolated by ODS column chromatography and high-performance liquid chromatography (HPLC) from hypocotyls of soybean.The inhibitory activities of each component of soyasaponins against α-glucosidase were tested by colorimetric method.Results:Soyasaponins showed potent inhibitory activities against α-glucosidase.Group B,group E and DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins showed stronger potency, which were non-competitive inhibitors of α-glucosidase with IC 50 values of 10～40 μmol/L. While group A saponins showed a little lower potency with IC 50 values of about 2 mmol/L.Conclusion:The results suggest soyasaponins,which exhibit inhibitory effects on α-glucosidase,seem physiologically useful for suppressing postprandial hyperglycemia in patients with diabetes mellitus.

关 键 词：大豆皂苷 Α-葡萄糖苷酶 抑制作用 研究 糖尿病 大鼠 

分 类 号：R285.5[医药卫生—中药学]