中国汉族、布依族和壮族群体中聚腺苷二磷酸核糖聚合酶-1基因多态性  

Human PARP-1 Gene Polymorphisms in Chinese Han, Buyi and Zhuang Populations

作　　者：唐焕文[1] 庄志雄[2] 梁海荣 李荣成[4] 何云[1] 农艺[4] 黄月葵[4] 

机构地区：[1]中山大学公共卫生学院卫生毒理学教研室,广东广州510080 [2]深圳市疾病预防控制中心,广东深圳518020 [3]广西灌阳县卫生防疫站,广西灌阳541600 [4]广西壮族自治区疾病预防控制中心,广西南宁530021

出　　处：《中山大学学报（医学科学版）》2003年第5期432-434,438,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家973计划资助课题(2002CB512904)

摘　　要：【目的】探讨人类聚腺苷二磷酸核糖聚合酶-1(PARP-1)5个外显子核苷酸多态性。【方法】采用聚合酶链式反应-单链构象多态性(PCR-SSCP)和银染技术检测3个民族(汉族、布依族和壮族)634名正常人PARP-1基因多态性。【结果】在3个民族634名正常人血标本的5个外显子扩增产物SSCP电泳条带中,219名汉族人PARP-1基因5个外显子均未检出多态性条带;203名布依族和212名壮族人PARP-1基因的5个外显子扩增产物中分别有2例的外显子20检出1条多态性条带,其余4个外显子扩增产物未见多态性条带。【结论】PARP-1基因第20外显子上可能存在多态性。Objective] To investigate nucleotide polymorphisms of five exons in human poly(ADP ribose)polymerase 1(PARP 1) gene. PCR SSCP silver staining was used to test the polymorphisms of PARP 1 gene in 634 chinese Han, Buyi and Zhuangzu people( 219, 203 and 212 respectively) . Polymorphisms of 5 exons of PARP 1 gene was not detected in 219 Han people and a polymorphisms of exon20 of PARP 1 gene was detected in 203 Buyi and 212 Zhuangzu people by PCR SSCP. Other exons were not detected. [ Conclusion] Polymorphisms may exist in exon 20 of PARP 1 gene.

关 键 词：汉族 布依族 壮族 聚腺苷二磷酸核糖聚合酶-1 基因多态性 聚合酶链式反应-单链构象多态性 银染技术 

分 类 号：R394[医药卫生—医学遗传学]