Cyclin D_1和p16、p27在脑胶质瘤中的表达及其与PCNA的相关性  

Relationship among expression of Cyclin D_1、p16、p27 and PCNA in glioma

作　　者：文志华[1] 赵时雨[1] 李志强[1] 陶红兵[1] 柯玮[1] 

机构地区：[1]武汉大学中南医院神经外科实验室,武汉430071

出　　处：《中国免疫学杂志》2003年第10期697-700,共4页Chinese Journal of Immunology

摘　　要：目的 :检测CyclinD1 和p16、p2 7以及PCNA在脑胶质瘤中的表达状况 ,以探讨不同病理类型脑胶质瘤中各自的表达及其相关性。方法 :应用免疫组化S P法 ,检测 12例正常脑组织、5 8例脑胶质瘤组织中CyclinD1 、p16、p2 7及PCNA表达及其特征。结果 :CyclinD1 在由低度恶性胶质瘤向高度恶性胶质瘤转化中阳性表达逐渐增强 (χ2 检验 ,P <0 0 0 5 ,χ2 =5 1 6 7) ;而p16、p2 7阳性表达却是随着胶质瘤恶性程度的升高而降低 (χ2 检验 ,P值均 <0 0 0 5 ,χ2 分别为 15 4 1和 12 81)。CyclinD1 与PCNA呈正相关 ,rs =0 74 5 ;p16和p2 7与PCNA呈负相关 ,rs分别为 - 0 5 6 6和 - 0 6 12。结论 :脑胶质瘤中CyclinD1的表达程度对胶质瘤的细胞增殖活性起促进作用 ,而p16、p2 7的表达则起抑制作用。Objective:To investigate the expression of Cyclin D 1?p16?p27 and PCNA in glioma with different pathological classification, and to explore the correlationship among Cyclin D 1?p16?p27 and PCNA.Methods:Cyclin D 1?p16?p27 and PCNA protein expressions in 12 normal brain tissues and 58 glioma cases were examined by immunohistochemical staining and hemiquantity analysis.Results:With the increasing of glioma malignancy, the expression level of Cyclin D 1 protein increased gradually (P<0.005, χ 2=51.67),while the expression lever of p16?p27 decreased (P<0.005 both, χ 2 is for 15.41 and 12.81,respectively).Positive correlation was found between Cyclin D 1 and PCNA (rs=0.745).There was negative correlation between p16?p27 and PCNA rs parting for -0.566 and -0.612.Conclusion:In the process of glioma cell proliferation,Cyclin D 1 may be facilitate, while p16 and p27 may be inhibitory.

关 键 词：胶质瘤 CYCLIN DL P16 P27 PCNA 相关性 

分 类 号：R739.41[医药卫生—肿瘤]