D-核糖产生菌转酮醇酶活性分析  

The Tansketolase Activity Determination of D-ribose-producing Strain

作　　者：谢红[1] 米丽娟[1] 孙文敬[1] 杨庆文[1] 蒋明珠[1] 

机构地区：[1]山西省生物研究所,山西太原030006

出　　处：《微生物学杂志》2003年第5期18-21,共4页Journal of Microbiology

摘　　要：芽孢杆菌属的转酮醇酶(Transketolase,TKT)变异株可利用培养基中的D-葡萄糖,将其转化成D-核糖分泌于细菌培养基中。阐述了D-核糖产生菌转酮醇酶的活性测定方法,制备了测定所必需的2种酶。Transketolase mutant of bacillus subtilis can produce D-ribose in medium using D-glucose as carbohydrate. We describe a method of transketolase activity determination, in the same time, we also purify two enzymes which were necessary for transketolase activity determination.

关 键 词：转酮醇酶 活性测定 D-核糖 产生菌 

分 类 号：TQ929[轻工技术与工程—发酵工程]