胡萝卜素漂白法快速筛选耐储藏水稻品种  

A Rapid Method for Screening Storable Rice Cultivars by Carotene Bleaching

作　　者：沈文飚[1] 俞伟伟[1] 汪仁[1] 江玲[1] 翟虎渠[2] 程式华[3] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程研究中心,江苏南京210095 [2]中国农业科学院,北京100081 [3]中国水稻研究所,浙江杭州310006

出　　处：《中国水稻科学》2003年第4期387-389,共3页Chinese Journal of Rice Science

基　　金：国家 8 6 3计划资助项目 ( 2 0 0 1AA2 4 1 0 1 9);科技部转基因专项资助项目 (J0 0 A0 0 6 1 )

摘　　要：根据水稻种胚脂氧合酶的主要同工酶LOX 3的活性缺失可以提高耐贮性的特性 ,研究了快速测定LOX 3酶活性以筛选耐储藏水稻品种的方法。报道了水稻种胚LOX 3具有共氧化的次生反应特性 ,并根据该特性建立了快速筛选其缺失体的胡萝卜素漂白法 (检测时间为 3h) ,优化了反应体系。推测在正常氧压下水稻种胚LOX 1和LOX 2不具备或仅具备相对较弱的共氧化次生反应能力。与LOX 3缺失体的单克隆抗体技术和I2 KI比色法相比 ,胡萝卜漂白法具有方法简单、成本低、快速的优点。Based on co-oxidation secondary reaction functio n of lipoxygenase-3(LOX-3) in rice embryo, a rapid method for detecting the lack of LOX-3 in rice embryos by carotene bleaching for only three hours was developed and optimized. The method is simple, inexpensive and suitable to rapid screening storable rice cultivars compared with monoclonal antibody technique a nd indide-starch method. It was deduced that LOX-1 and LOX-2 in rice embryos probably displayed less or no co-oxidation activities under normal air conditi on in comparison with that of LOX-3. The perspective of carotene bleaching method on the usage in breeding for stora ble rice cultivars and related-cloning research was also discussed.

关 键 词：胡萝卜素漂白法 筛选 耐储藏品种 种胚 脂氧合酶 同工酶缺失体 水稻 

分 类 号：S511[农业科学—作物学]