单向薄层色谱技术分离磷脂的方法研究  

IMPROVED ONE-DIMNSIONAL THIN-LAYER CHROMATOGRAPHIC SEPARATION OF EGG PHOSPHOLIPIDS

作　　者：孙秀燕[1] 宋爱菊[2] 张治国[3] 

机构地区：[1]沈阳药学院测试中心 [2]沈阳药学院药学系 [3]中国人民解放军81945部队

出　　处：《沈阳药学院学报》1992年第4期262-265,共4页

摘　　要：本文采用氯仿-甲醇-醋酸盐缓冲液(pH3.8)(65-25-3.5)为流动相,碱性硅胶H为固定相,对制备的新型药用亲油性表面活性剂的蛋黄磷脂各组分进行了定性定量分析,并对磷脂定性分析方法进行了探讨。Described here is the separation of egg phospholipids on a basic silica gel H plate developed with chloroform-methanol-acetate buffer solution (pH 3.8) (65:25:3.5). The method was used effectively to separate phosphatidylcholine (PC), leso-phosphatidylcholine (LPC), leso-phosphatidylethanolamine (LPE), phosphatidylethanolamine (PE), phosphatidylinositol (PI), sphingomyelin (SM), cholesterine (Chol), and vitamine E(V_E) in thesamples tested. The method was also applied qualitatively.

关 键 词：薄层色谱法 蛋黄磷脂 醋酸盐缓冲液 

分 类 号：R914.1[医药卫生—药物化学]