第56位酪氨酸与肿瘤坏死因子细胞毒活性的关系  

Significance of the 56th Tyrosine of Tumor Necrosis Factor for Its Cytotoxic Activity

作　　者：张德震[1] 陈苏民[1] 陈南春[1] 智刚[1] 苏成芝[1] 

机构地区：[1]第四军医大学生物化学教研室

出　　处：《生物工程学报》1992年第1期23-27,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金

摘　　要：选择肿瘤坏死因子分子中间部分保守区第56位酪氨酸,用寡核苷酸诱导的体外基因定点突变法,将其改变为谷氨酰胺。将突变的肿瘤坏死因子基因插入表达载体pBV220,所得表达产物的分子大小及表达量均无明显变化,但其细胞毒活性及比活性均下降数百倍,表明该区是肿瘤坏死因子细胞因子细胞毒活性的必需区。A conserved amino acid residue, the 56th tyrosine was substituted by glutamine using site-specific mutagenesis. The expression level of this mutant protein in E. coil did not changed signifigantly, however, its specific activity of the cytotocity on L929 cultural cells decreased 583 times as-comparing to the unmutant tumor necrosis factor. The results show that the tyrosinc 56 residue or the region where tyrosine 56 located is important for the cytotoxicity of tumor necrosis fator.

关 键 词：肿瘤坏死因子 酪氨酸 细胞毒活性 

分 类 号：R394.8[医药卫生—医学遗传学]