枯草杆菌α-淀粉酶基因的克隆和表达  

Cloning and Expression of α-amylase from Bacillus subtilis in Escherichia coli and Bacillus subtilis

作　　者：熊占[1] 涂祖新[1] 周和山[1] 黄筱萍[1] 

机构地区：[1]江西科学院微生物研究所

出　　处：《生物工程学报》1992年第4期334-338,共5页Chinese Journal of Biotechnology

摘　　要：以自构的质粒pBE1为载体,采用鸟枪法克隆枯草杆菌168突变株GR10的α-淀粉酶基因,获得了在大肠杆菌中的表达。该基因片段位于7367bp的Bgl Ⅱ酶切片段上。利用质粒pUB110将此片段亚克隆到枯草杆菌中,同样也获得了表达。本文还测定了该基因克隆子是否有GR10的抗葡萄糖阻遏效应。With a mutant strain of Bacillus subtilis 168 as a donor and plasmid pBE1 constructed by ourselves as a vector, the a-amylase gene was successfully cloned and expressed in E. coli by means of Shutgun method. This α-amylase gene was situated in 7367 bp Bgl Ⅱ restriction fragment, which was then subcloned and expressed in Bacillus subtilis by using plastaid pUB110 as a vector. It was also examinated whether the fragment mediated glucose repression resistance of GR10 to the synthesis of a-amylase in this paper.

关 键 词：基因克隆 Α-淀粉酶基因 大肠杆菌 

分 类 号：Q81[生物学—生物工程]