应用PCR及寡核苷酸探针研究人原发性肺癌中Ki-ras点突变  

MUTATION OF Ki-ras ONCOGENE IN HUMAN LUNG CANCER DETECTED BY POLYMERASE CHAIN REACTION (PCR) AND OLIGONUCLEOTIDE HYBRIDIZATION

作　　者：覃扬[1] 孙芝琳[1] 赵建升 孙宁[1] 步宏[2] 刘开凤[2] 

机构地区：[1]华西医科大学生化教研室,成都610041 [2]华西医科大学病理教研室

出　　处：《生物化学与生物物理进展》1992年第4期277-281,共5页Progress In Biochemistry and Biophysics

基　　金：国家"七.五"攻关资助项目

摘　　要：应用PCR及寡核苷酸探针杂交方法研究了50例人原发性肺癌石蜡切片标本 中Ki-ras 12(Val),Ki-ras 13(Cys)点突变,发现13例为点突变阳性,突变率为26％,其中3例同时兼有二种点突变(Val/Cys);Ki-ras 12位的点突变率(20％)比Ki-ras 13位的点突变率(12％)高。同时还发现Ki-ras基因的点突变存在于腺癌、鳞癌、小细胞肺癌以及各种分化程度的肺癌中。The frequency of oncogene Ki-ras12 (Val), Ki-ras13 (Cys) mutation was studied in paraffin embedded sections obtained from 50 human lung cancer by in vitro amplification of target sequences via polymerase chain reaction (PCR)and selective oligonucleotide hybridization. 13 samples (26%) contained Ki-ras mutation. 3 carcinomas exhibited 2 distint Ki-ras mutation. The Ki-ras mutation was present in adenocarcinoma, squamous cell carcinoma, adenosquamous carcinoma, well differentiated, moderately differentiated, poorly differentiated human lung cancers.

关 键 词：肺肿瘤 癌基因 点突变 PCR 

分 类 号：R734.202[医药卫生—肿瘤]