EFFECTS OF XW630 ON BONE FORMATION IN OVARIECTOMIZED RATS AND IN HUMAN OSTEOBLAST-LIKE CELLS  

作　　者：孙兰 邱明才 翁玲玲 郑虎 刘景生 

机构地区：[1]DepartmentofPharmacology,InstituteofBasicMedicalSciences,CAMS＆PUMC,Beijing100005 [2]DepartmentofEndocrinology,TianjingMedicalUniversityHospital,Tianjing [3]PharmacyCollege,WestChinaUniversityofMedicalSciences,Chengdu

出　　处：《Chinese Medical Sciences Journal》2000年第1期8-13,共6页中国医学科学杂志（英文版）

基　　金：This project was supported by the National Natural ScienceFoundation of China;No.39430 120

摘　　要：To study the effects of XW630 on bone formation in overiectomized(OVX) rats and in human osteoblast like cell line TE85. [WT5”BX]Method.[WT5”BZ] Bone histomorphometric analysis was performed with undecalcified bone sections and tetracycline intraperitoneally labeling. [WT5”BX]Results.[WT5”BZ] Compared with that of OVX rats, the static data of trabecular bone volume(TBV)/ total tissue volume(TTV), TBV/sponge bone volume(SBV) and mean trabecular plate density (MTPD) were enhanced while mean trabecular plate spacing(MTPS) decreased after treated with XW630 for 13w. The dynamic data of single labeled surface [Sfract(s)], double labeled surface[Sfract(d)],Sfract(d+1/2s),trabecular osteoid surface(TOS), and bone formation rate in tissue level (Svf) were increased and osteoid maturation period (OMP) shortened in XW630 group. In osteoblast like cells, both 3H thymidine incorporation and cell count increased after treated with XW630 for 48. Treated with XW630 for 12～18h,inducible nitric oxide synthase(iNOS) activity and cGMP content increased in time dependent manners. [WT5”BX]Conclusions.[WT5”BZ] XW630 enhanced bone activation frequency and increased trabecular connectivity, stability, and strength. The cellular mechanism related to effects of XW630 on bone formation in ovariectomized rats.

关 键 词：WT5BZ]XW630 OVARIECTOMY OSTEOBLAST 

分 类 号：R588[医药卫生—内分泌]