中国人血管抑素基因的克隆及其序列分析  

Cloning and sequencing of Chinese human angiostatin

作　　者：范天黎[1] 姜国忠[1] 牛向丽[1] 谢华[1] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第6期881-884,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目 3 0 2 70 0 3 1;河南省重大科技攻关基金资助项目 0 12 2 0 3 2 5 0 0 ;河南省杰出人才创新基金资助项目 0 2 2 10 0 190 0

摘　　要：目的 :克隆中国人血管抑素 (angiostatin ,ANG)基因全长并进行序列分析。方法 :应用RT -PCR ,将 5例健康人肝脏组织ANG基因克隆到pSP71载体上 ,用Sanger法进行基因测序分析。结果 :通过RT -PCR获得一个 1 1 4 1bp的扩增产物 ,测序发现与已报道的ANG比较 ,国人ANG上有 3个碱基突变位点 :分别为第 82 5位C→T ;第 92 7位T→G和第 1 0 78位G→A。前两个碱基突变氨基酸未发生变化 ,第 3个碱基突变结果使缬氨酸 (Val342 )改变成为蛋氨酸(Met342 )。结论 :克隆得到中国人ANG基因片段 。Aim:To clone and sequence the full-length angiostatin from Chinese plasminogen. Methods:Chinese angiostatin gene was cloned on the Xho I and EcoR I sites of pSP71 vector by RT-PCR, and then sequenced by using Sanger method through sp6 and T7 promoter primers. Results:A recombinant plasmid pSP-hANG was constructed. Three mutant sites were detected compared with the reported plasminogen cDNA sequence, which were 825 site (C →T), 927 site(T→G) and 1 078 site(G →A),respectively. No amino acid change occurred in the first two mutant sites, but amino acid Val342 was superseded by Met342 in the third mutant site. Conclusion:The variety of base-pair and amino acid in the Chinese angiostatin might involve in genetic polymorphism.

关 键 词：血管抑素 纤溶酶原 遗传多态性 

分 类 号：Q52[生物学—生物化学]