胶质瘤细胞ING1、人端粒酶逆转录酶及人端粒酶相关蛋白1基因表达的研究  

Study on expression of ING1, human telomerase reverse transcriptase and telomerase-associated protein 1 genes in human gliomas

作　　者：于士柱[1] 黄悦[2] 李莉[3] 管欣琴[1] 张丽侠[1] 安同岭[1] 

机构地区：[1]天津医科大学总医院,天津市神经病学研究所300052 [2]天津医科大学眼科中心,300070 [3]天津大港油田职工总医院脑系科,300280

出　　处：《中华病理学杂志》2003年第3期215-219,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金(39870335)

摘　　要：目的 探讨胶质瘤细胞ING1、人端粒酶逆转录酶(hTERT)和人端粒酶相关蛋白1(hTP1)基因表达的意义。方法 用mRNA原位杂交及免疫组织化学染色方法观察了70例不同级别的人胶质瘤组织。结果 hTERT mRNA和蛋白的阳性表达率分别为88.6％和82.9％,hTP1 mRNA和蛋白的阳性表达率均为100％,这4种阳性肿瘤细胞的密度彼此间均呈正相关(r=0.758～0.882,P<0.0005),并均随肿瘤级别升高而相应增加(P<0.05～0.01)。ING1 mRNA和蛋白的阳性表达率分别为94.3％和88 6％,两种阳性肿瘤细胞密度间也呈正相关(r=0.831,P<0.0005),但两者均随肿瘤级别升高而相应减少(P<0.01),并均分别与hTERT mRNA和蛋白及hTP1 mRNA和蛋白的阳性肿瘤细胞密度呈负相关(r=-0.211～-0.384,P<0.05～0.001)。结论 胶质瘤细胞中hTERT和hTP1基因表达异常增加可能是抑制其ING1基因表达的重要因素,这3种基因的表达异常可能在胶质瘤发生及恶性进展过程中起重要作用。Objective To investigate the relationship between expressions of ING1 gene and genes of human telomerase reverse transcriptase ( hTERT ) and telomerase-assoeiated protein 1 ( hTP1 ) in human gliomas. Methods The expressions of ING1 mRNA and p33ING1 protein, hTERT mRNA and protein, and hTP1 mRNA and protein in seventy human glioma specimens with different malignant grades were studied using in situ hybridization and immunohistochemistry. Results All of the 70 gliomas collected expressed hTP1 mRNA and protein and among them, 62 (88.6% ) and 58 (82.9% ) out of 70 expressed hTERT mRNA and protein respectively. The quantities of the four kinds of positive cells were correlated positively with one another (r =0. 758-0. 882, P <0. 000 5) , and all of them were significantly fewer in gliomas of WHO grade Ⅰ-Ⅱ than in grade Ⅲ gliomas and the most in grade Ⅳ gliomas (P < 0. 05-0.01). 66 (94.3%) and 62 (88.6%) out of 70 gliomas expressed ING1 mRNA and p33ING1 protein respectively. The quantities of their positive cells were also correlated positively with each other (r = 0. 831 , P < 0. 0005) , but the positive cells were more in gliomas of WHO grade Ⅰ-Ⅱ than in grade Ⅲ gliomas and the fewest in grade Ⅳ gliomas (P <0. 01). The quantities of positive cells of ING1 mRNA and p33ING1 protein were correlated negatively with those of hTERT mRNA and protein as well as hTP1 mRNA and protein respectively (r= -0.211--0.384, P < 0. 05 -0.001). Conclusions The results suggest that all of the parameters concerned are valuable in evaluating the biological behavior of gliomas. In glioma cells, overexpressions of hTERT and hTP1 genes might be significant in inhibiting the expression of ING1 gene. The abnormal expressions of the three genes play possibly the important roles in the development and malignant progression of gliomas.

关 键 词：胶质瘤细胞 INGl 端粒酶 逆转录酶 蛋白1基因 表达 研究 免疫组织化学 染色方法 

分 类 号：R730.2[医药卫生—肿瘤]