SARS冠状病毒N蛋白单克隆抗体在SARS尸检组织中的表达  

Expression of the monoclonal antibody against nucleocapsid antigen of SARS-associated coronavirus in autopsy tissues from SARS patients

作　　者：贺莉[1] 丁彦青[1] 车小燕[2] 张庆玲[1] 黄仲曦[1] 王慧君[1] 申洪[1] 李祖国[1] 蔡俊杰[1] 张进华[1] 耿舰[1] 李欣[1] 张文丽[1] 韩慧霞[1] 康伟[1] 杨磊[1] 陆药丹[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515 [2]第一军医大学珠江医院实验中心,广东广州510515

出　　处：《第一军医大学学报》2003年第11期1128-1130,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(30340015);军队医药卫生SARS专项基金(03F016-2);广东省SARS专项基金(GD2003-80)~~

摘　　要：目的观察SARS死者的尸检组织中SARS冠状病毒(SARS-CoV)的存在与分布情况。方法应用免疫组化技术检测4例尸检标本肺、脾脏、淋巴结、脑、垂体、心、肝、肾、胰腺、气管、食道、胃肠道和骨髓等组织的SARS-CoV N蛋白。结果肺泡上皮、肺、脾、淋巴结内浸润的单核细胞/巨噬细胞、脑神经元、肝细胞、肾远曲小管上皮细胞、胰腺腺泡细胞、垂体嗜酸细胞、甲状旁腺嗜酸性细胞、肾上腺皮质细胞、气管及支气管浆液腺上皮细胞、食道粘膜鳞状上皮、胃肠道柱状上皮细胞及胃壁细胞、骨髓早幼粒细胞及小静脉内皮细胞等细胞质内SARS-CoV N蛋白单克隆抗体均为阳性。结论SARS-CoV可侵犯全身多种器官和组织。SARS-CoV N蛋白单克隆抗体在胃肠道、肾远曲小管及汗腺细胞内的阳性表达,对研究SARS-CoV传播途径具有重要意义。Objective To investigate the presence and distribution of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in autopsy tissues obtained from patients died of SARS. Methods Immunohistochemical technique was applied in 4 fatal SARS cases to examine the autopsy tissues including the lungs, spleen, lymph nodes, brain, pituitary, heart, liver, kidney, pancreas, trachea, esophagus, gastrointestinal tract, adrenal glands, parathyroids, skin and bone marrow. Results Immunohistochemistry identified positive monoclonal antibody against SARS-CoV nuceeocapsid (N) protein in the alveolar epithelium and the infiltrating monocytes or macrophages in the lung, spleen and lymph nodes; the presence of the antibody was also detected in the serous gland epithelium of the trachea/bronchus, squamous epithelium of the esophagus, the gastric parietal cells, the epithelium of the intestinal tract, acidophilic cells in the parathyroids and pituitary, acinus cells in the pancreas, adrenal cortical cells, sweat gland cells, small vessel endothelium, bone marrow promyelocytes, epithelial cells of the distal convoluted tubule of the kidney, brain neurons, and the hepatocytes near the central vein. Conclusion A variety of organs and tissues can be infected by SARS-CoV, and the positive expression of SARS-CoV N protein in the epithelial cells of the gastrointestinal tract, the distal convoluted tubule of the kidney and the sweat gland cells is significant for studying the transmission routes of SARS.

关 键 词：SARS冠状病毒 N蛋白单克隆抗体 尸检组织 SARS-COV 急性重症呼吸综合征 

分 类 号：R361.2[医药卫生—病理学]