Glucosides of Chaenomeles speciosa remit rat adjuvant arthritis by inhibiting synoviocyte activities  

木瓜苷通过抑制滑膜细胞功能减轻大鼠佐剂性关节炎(英文)

作　　者：戴敏[1] 魏伟[2] 沈玉先[2] 郑咏秋[2] 

机构地区：[1]安徽中医学院药学系 [2]安徽医科大学临床药理研究所

出　　处：《Acta Pharmacologica Sinica》2003年第11期1161-1166,1176,共7页中国药理学报（英文版）

基　　金：Project supported by the Natural Science Foundation of Anhui Province, China, № 00044411.

摘　　要：AIM: To investigate the effects of Glucosides of Chaenomeles speciosa(GCS) on rat adjuvant arthritis(AA) and to clarify the role of synoviocytes in this process. METHODS: Complete Freund's adjuvant was used to induce AA in rats. Secondary paw swelling of AA rats was measured with MK-550 volume meter. The pain response and polyarthritis index were scored. Synoviocytes were separated by incubation of collagenase and trypsin, and morphological changes of synoviocytes were observed by transmission electron microscope. Interleukin-1(IL-1) production was measured by thymocyte proliferation assay. Tumor necrosis factor(TNFα) and prostaglandin E_2(PGE_2) production were determined by radioimmunoassay. RESULTS: There were significant secondary inflammatory reactions in AA rats. The morphology of synoviocytes from AA rats was changed, companying the elevation of the level of IL-1,TNFα, and PGE_2 produced by synoviocytes from AA rats. GCS(60 and 120mg/kg, ig, 8d) suppressed secondary inflammatory paw swelling, pain response, and polyarthritis index. It also improved ultrastructural changes of synoviocytes and inhibited IL-1,TNFα, and PGE_2 production in AA rats. The inhibitory effect of GCS 120mg/kg was more evident than that of Actarit 60mg/kg. CONCLUSION: GCS reduced the secondary inflammatory in AA rats, which is associated with prevention of ultrastructural changes of synoviocytes and inhibition of secretion of proimflammatory cytokines.目的:观察木瓜苷(GCS)对大鼠佐剂性关节炎(AA)的作用,分析该作用与改善滑膜细胞的形态和功能之间的联系。方法:弗氏完全佐剂诱导大鼠AA,足容积法测量AA大鼠继发侧足容积,对关节疼痛和多发性关节炎进行评分,用Ⅱ型胶原酶和胰蛋白酶消化和分离滑膜细胞,透射电镜进行滑膜细胞的超微结构观察,小鼠胸腺细胞增殖法测定滑膜细胞培养上清液中的IL-1水平,放射免疫法测定其中TNFα和PGE_2含量。结果:GCS(60,120mg/kg)连续灌胃8天,可明显减轻大鼠AA的继发性足肿胀、炎性疼痛反应和多发性关节炎指数,改善滑膜细胞形态的异常并降低其高分泌的IL-1、TNFα和PGE_2含量;GCS(120mg/kg)在减轻继发性炎症、疼痛和抑制滑膜细胞分泌PGE_2方面明显优于阿克他利。结论:GCS具有减轻大鼠AA的作用,该作用的发挥可通过改善滑膜细胞异常的超微结构并抑制其过度分泌IL-1。

关 键 词：Chaenomeles speciosa GLUCOSIDES immunologic adjuvants experimental arthritis synovial membrane INTERLEUKIN-1 tumor necrosis factor prostaglandins E 

分 类 号：R285[医药卫生—中药学]