一种简单高效的食用真菌总RNA提取方法  

A Simple and Effective Method for Extracting Total RNA from Domestic Fungus

作　　者：周凯松[1] 薛久刚[1] 常宁[1] 陈畅[1] 张晗星[1] 张长铠[1] 

机构地区：[1]山东大学生命科学学院微生物技术国家重点实验室,济南250100

出　　处：《遗传》2003年第6期703-704,共2页Hereditas（Beijing)

基　　金：高等学校博士学科点专项科研基金(2000042212)资助

摘　　要：以金针菇为材料,建立了一种适合于富含RNase、多酚、多糖和糖蛋白的食用真菌RNA的提取方法,此方法在高浓度变性剂存在的条件下2次用苯酚 氯仿 异戊醇进行抽提去除DNA、蛋白质,并用异丙醇和乙酸钠选择性沉淀RNA、去除多糖,得到完整、均一的RNA样品。With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method.

关 键 词：食用菌 RNA提取 金针菇 提取方法 

分 类 号：Q943[生物学—植物学]