The Sequestered Actin Was Bound to Non-actin Protein(s) in the ATP-depleted Rabbit Proximal Tubules  

在兔近端小管ATP缺乏时部分肌动蛋白与非肌动蛋白相结合(英文)

作　　者：顾洛[1] 杜军[1] 戈应滨[1] 范乐明[2] 陈琪[2] 陈进[3] 

机构地区：[1]南京医科大学生理学系 [2]南京医科大学病理生理学系 [3]印第安那州立大学生命科学系

出　　处：《Journal of Nanjing Medical University》2003年第6期270-276,共7页南京医科大学学报（英文版）

基　　金：National Institute of Diabetes and Digestive and Kidney Diseases Grant R15 DK56599-01 to CHEN Jin

摘　　要：Objective: To demonstrate whether the actin breakdown fromactin-cytoskele-ton in microvilli was bound to any non-actin protein ( s ) in 'ATP-depleted rabbitproximal tubules (PT). Methods: By using DTSSP to crosslink actin and non-actin protein (s) ininsoluble fraction of ATP-depleted PT, the crosslinked protein complexes were analysed bytwo-dimensional electrophoresis and western blotting. Results: Analysis of the crosslinked actincomplexes revealed one complex that contained actin and non-actin protein. Villin was not detected.Conclusion: The results strongly suggested that some of the sequestered actin was directly bound tonon-actin protein (s) other than was co-precipitated with villin or the nuclei as hydrophobic actinhomopolymers.目的:研究的目的是阐明在ATP缺乏的免近端小管(PT)中,自微绒毛内肌动蛋白细胞骨架上崩解的肌动蛋白是否与非肌动蛋白结合。方法:首先用DTSSP交联存在于ATP缺乏的PT不溶组分中肌动蛋白和非肌动蛋白结合的方法,交联复合物进一步用二维电泳及免疫印迹法分析。结果:分析被交联的肌动蛋白复合物发现肌动蛋白与非肌动蛋白结合,而并非与绒毛蛋白结合。结论:本结果首次提示:在ATP缺乏的免PT中,源于肌动蛋白细胞骨架的部分肌动蛋白是与非肌动蛋白相结合,而不与绒毛蛋白直接结合或以疏水性共聚体与核共存的。

关 键 词：ACTIN VILLIN ISCHEMIA MICROVILLI kidney 

分 类 号：R363[医药卫生—病理学]