L-缬氨酸产生菌的选育及其代谢流分析  

Breeding of L-valine Producer and Its Metabolic Flux Analysis

作　　者：李秀敏[1] 杨毅[1] 姜绪林[1] 张星元[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《生物技术》2003年第6期19-21,共3页Biotechnology

摘　　要：以谷氨酸棒杆菌 (Corynebacteriumglutamicum)AS1.4 95 (Leu- )为出发菌株 ,通过多次亚硝基胍 (NTG)诱变 ,给AS1.4 95 (Leu- )依次叠加L -AAHss,2 -TAr,Vd- 的遗传标记 ,得到突变株AATV341(Leu- ,L -AAHss,2 -TAr,Vd- ) ,可在 8%的葡萄糖培养基积累L-缬氨酸 2 4 .5g L ,比出发菌株提高了 5 .13倍。同时运用代谢流量分析理论 ,测定出发菌株AS1.4 95及其突变株AATV341在L -缬氨酸合成阶段的代谢流量 ,并初步进行比较和分析 ,发现遗传标记的引入使流量分配发生了重大变化 ,流量分配朝着有利于L -缬氨酸合成的方向改变。AATV341(Leu^-,L-AAH^(ss),2-TA^r,Vd^-) was obtained by N-methyl-N'-nitro-N-nitroso-guanidine (NTG) mutagenesis derived from the original strain Corynebacterium glutamicum AS1.495(Leu^-).AATV341 can produce 25.4 g/L of L-valine in 8% glucose medium,which increased 5.13 times more than AS1.495.According to the theory of metabolic flux analysis,the metabolic flux of Corynebacterium glutamicum AS1.495 and its mutants AATV341 was determined,compared and analyzed during L-valine synthesis.The metabolic flux distribution of AS1.495 changed greatly,which was caused by the introduction of genetic markers and was useful to L-valine synthesis.

关 键 词：L-缬氨酸 产生菌 选育 代谢流分析 谷氨酸棒杆菌 亚硝基胍诱变 

分 类 号：TQ922[轻工技术与工程—发酵工程]