种子贮藏蛋白凝胶电泳在小麦外源染色体鉴定中的应用  

Application of proteins gel electrophoresis in the identification of allosome chromosome in wheat

作　　者：王小利[1] 张改生[1] 陈新宏[1] 赵继新[1] 刘宏伟[1] 王军卫[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2004年第1期19-22,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30170567);杨凌农业生物技术育种中心基金项目

摘　　要：　利用小麦种子贮藏蛋白凝胶电泳技术,对簇毛麦(Haynaldiavillosa)、滨麦(Leymusmollis)、华山新麦草(Psathyrostachyshuashanica)、大麦(Hordeumdistichom)以及黑麦(Secalecereale)的高分子量谷蛋白亚基和醇溶蛋白进行了分析。结果表明,簇毛麦、滨麦、华山新麦草、大麦的高分子量谷蛋白亚基和醇溶蛋白与普通小麦相比均有各自的特征带;利用簇毛麦和大麦的高分子量谷蛋白亚基特征带鉴定出了普通小麦中与小麦第一同源群染色体有部分同源的V1和H5附加系;利用黑麦1RS醇溶蛋白标记位点Gld1B3鉴定了1BL/1RS易位系,同时利用染色体C-分带对1BL/1RS易位系77(2)进行了验证。并对小麦种子贮藏蛋白凝胶电泳技术在小麦品质育种和细胞质雄性不育选育中的应用进行了讨论。Wheat proteins gel electrophoresis was used to identify allosome chromosome of Haynaldia villosa,Leymus mollis,Psathyrostachys huashanica,Hordeum distichom and Secale cereale in wheat.All of these plants had their own HMW-GS and gliadin marker,compared to wheat.V1 and H5 additional lines were detected by HMW-GS marker of SDS-PAGE,which have partial homologous relations with the first homologous series chromosome in wheat.1BL/1RS translocation lines was detected with the gliadin marker Gld1B3 of 1RS translocation chromosome and C-banding.Meantime,the technology application of wheat proteins gel electrophoresis was discussed in quality control and male sterile breeding of wheat.

关 键 词：种子贮藏 蛋白凝胶电泳 小麦 外源染色体 鉴定技术 谷蛋白亚基 醇溶蛋白 

分 类 号：S512.1[农业科学—作物学]