肝细胞癌患者的谷胱甘肽硫转移酶基因缺失的研究  

Genetic polymorphisms of Glutathione S-transferase M1 and T1 and the risk of hepatocellular carcinoma

作　　者：韦义萍[1] 马韵[2] 邓卓霖[2] 

机构地区：[1]广西医科大学护理学院病理学教研室,南宁530021 [2]广西医科大学病理学教研室

出　　处：《肿瘤》2003年第6期464-466,共3页Tumor

基　　金：.国家自然科学基金资助项目 (编号 3 9860 0 3 2 );广西教育厅重点资助课题基金 (编号 98 2 8)

摘　　要：目的　研究广西黄曲霉毒素B1(AFB1)高污染区人群谷胱甘肽硫转移酶GSTM 1和GSTT1基因缺失多态性与肝细胞癌高发的相关性。方法　采用聚合酶链反应 (PCR)方法检测 110例高污染区肝细胞癌患者和 135例无癌健康对照者的GSTM1和GSTT1基因型分布。结果　正常对照组GSTM 1和GSTT1基因缺失率分别为 4 7.4 %和 4 0 .7% ,肝细胞癌组GSTM 1和GSTT1基因缺失率分别为 6 3.6 %和 6 0 .0 % ;GSTM 1基因缺失在肝细胞癌组和正常对照组之间 ,差异具有显著性意义 (P <0 .0 5 ) ;GSTT1基因缺失在肝细胞癌组和正常对照组之间差异具有非常显著性意义 (P <0 .0 1)。结论　肝细胞癌患者GSTM1和GSTT1基因缺失水平偏高可能是易感的原因之一。Objective To study the relationship between the genetic polymorphisms of Glutathione S-transferase (GST) M1 and TI and the hepatocellular carcinoma (HCC) at aflatoxin B 1 high risk region in Guangxi. Methods A multiplex polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in blood samples. The studied groups in the AFB 1 high risk region included 110 HCC cases identified by pathologist and 135 normal adults without any cancer Results The frequencies of GSTM1-null and GSTT1-null genotype in the control group were 47.4% and 40.7% , while in HCC group the frequencies were 63.6% and 60.0% respectively. The differences between GSTM1-null for control vs HCC and that between GSTM2-null for control vs HCC are P<0.05 and P<0.01 respctively. Conclusion The GSTM1-null genotype and GSTM1-null genotype are common in population living in an AFB 1 risk region in Guangxi. GSTM1 and GSTT1 genetic deletion may cause the residents predisposed to HCC.

关 键 词：肝细胞癌 谷胱甘肽硫转移酶 基因缺失 黄曲霉毒素B1 

分 类 号：R735.7[医药卫生—肿瘤]