Protective effect of ginsenoside Rg1 on dopamine-induced apoptosis in PC12 cells  

人参皂甙Rg_1对抗多巴胺诱导的PC12细胞凋亡(英文)

作　　者：陈晓春[1] 朱元贵 王小众 朱理安 黄春 

机构地区：[1]福建医科大学附属协和医院福建省老年医学研究所,福州中国350001

出　　处：《Acta Pharmacologica Sinica》2001年第8期673-678,共6页中国药理学报（英文版）

基　　金：Project supported by Fujian Natural Science Foundation,No 982004

摘　　要：AIM: To explore the possible molecular mechanism of exogenous dopamine-induced apoptosis in PC12 cells and the protective effect of ginsenoside Rg1. METHODS: Flow cytometric assay was used to quantify the apoptotic cells and measure the percentage of cells with positive Bcl-2 and Bax proteins. The morphology of apoptotic cells was evaluated by transmission electron microscope. DNA fragmentation was observed by gel electrophoresis. Caspase-3 activity was determined by fluorescent spec-trofluorometer and the expressive bcl-2 and bax mRNA by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Dopamine 0.15, 0.30, 0.45, and 0.60 mmol/L induced PC12 cell apoptosis from 1.1 % ± 0.4 % (control) to 41 % ± 3 %, 46.4 %±2.7 %, 53 %±3 %, and 64.5 %±2.7 %, respectively. After treatment with dopamine 0.45 mmol/L following pretreatment with Rgl 10 μmol/L for 24 h, the percentage of apoptotic cells and caspase-3 activity decreased from 53 % ± 3 % and 683 ± 8 (mean fluorescence intensity, MFI) to 1.9 %±0.6 % and 325 ± 5, and the percentage of cells with positive Bcl-2 protein increased from 14.3 % + 1.1 % to 25.9 % ± 1.6 %, however, the percentage of cells with positive Bax protein decreased from 48 % ± 3 % to 35 % ± 3 % , compared with group treated with DA 0.45 mmol/ L alone. CONCLUSION: Ginsenoside Rgl protectedPC12 cells against apoptosis by inhibiting the activation of caspase-3 and regulating the ratio of Bcl-2 to Bax protein.目的:探讨外源性多巴胺诱导PC12细胞凋亡以及人参皂甙Rg1保护作用的分子机制。方法:流式细胞仪定量测定PC12细胞的凋亡和Bcl-2、Bax蛋白的表达;电子显微镜观察PC12细胞的形态;凝胶电泳评价DNA的断裂;荧光分光光度计法测定caspase-3的活力;半定量RT-PCR分析bcl-2和bax mRNA的表达。结果:多巴胺(浓度为0.15、0.30、0.45和0.60mmol/L)诱导PC12细胞凋亡,各剂量组细胞凋亡率分别从对照组1.1％±0.4％增加到41％±3％,46.4％±2.7％,53％±3％和64.5％±2.7％;人参皂甙Rg1 10μmol/L预处理24h后,较多巴胺0.45mmol/L单独处理时,PC12细胞的凋亡率和caspase-3的活力分别从53％±3％和683±8(平均荧光强度)下降到1.9％±0.6％和325±5,Bcl-2蛋白阳性率从14.3％±1.1％增加到25.9％±1.6％,Bax蛋白阳性率从48％±3％下降到35％±3％。结论:人参皂甙Rg1通过抑制cas-pase-3的激活并调节Bcl-2和Bax两者间蛋白的比值对抗多巴胺对PC12细胞凋亡的诱导作用。

关 键 词：GINSENG SAPONINS DOPAMINE APOPTOSIS PC12 cells bcl-2 genes 

分 类 号：R285[医药卫生—中药学]