^(188)Re辐射诱导食道组织Caspase基因表达研究  

CASPASE GENE EXPRESSION IN ESOPHAGEAL TISSUE INDUCED BY ^(188)Re

作　　者：赵涤非[1] 楚建军[1] 胡玉林[1] 王震吾[1] 王明智[1] 许敏[1] 

机构地区：[1]江苏省无锡市第四人民医院,江苏无锡214062

出　　处：《实用临床医药杂志》2003年第5期450-452,共3页Journal of Clinical Medicine in Practice

摘　　要：目的　通过观察1 88Re辐射诱导食道组织Caspase基因表达 ,探讨β源核素的辐射效应机制 ,为1 88Re食道支架的临床应用提供参考依据。方法　免疫组化M30 检测法观察动物食道腔内近距离辐射 7d后Caspase活化情况及其凋亡发生率。结果　1 88Re 12mci支架腔内照射后食道M30 阳性标本检出率 3 7 5 0 % (6/2 4) ,显著高于其邻近组织气管 8 3 3 % (1/12 )和主动脉 7 14 % (1/14 )。在 6mci照射后的食道组织其AIM30 阳性表达的细胞凋亡指数为 (6 62± 1 3 4) % ,12mci为 (12 41±2 5 6) % ,后者显著高于前者 (P <0 0 1)。结论　1 88Re食道支架 12mci可诱导食道组织发生大量细胞凋亡。Caspase基因参与调控细胞凋亡过程。β源核素近距离照射对食道组织产生明显辐射效应 。Objective: To investigate the mechanism of radiation effect of β?ray nuclide by observing the caspase gene expression in the esophageal tissue induced by 188 Re radiation. Methods: The activation of caspase and the occurrence rate of apoptosis of animal esophagus 7d after introcavitary brachytherapy were observed. the immuno by using histo?chemistry assay M 30 (the caspase gene expression product). Results: In the 188 Re 12 mci sent group the M 30 positive was 37 5%(6/24) significantly higher than the nearby tracheal tissue 8 33%(1/12) and aorta 7 14%(1/14). The apoptosis rate index of esophageal tissue after 6mci radiation was (6 62±1 34)%. Where′as after 12 mci radiation (12 41±2 56)%, P<0 01. Conclusions: 188 Re esophageal sent with 12 mci may induce sufficient amount of cell apoptosis and caspase gene are related to the modulation of apoptosis. The brachytherapy of β?ray nuclide esophageal tissue has evident radiobiological effect and little harmful effect to the neighboring normal tissue.

关 键 词：^188RE CASPASE 细胞凋亡 辐射诱导 食道组织 基因表达 免疫组化 

分 类 号：R730.55[医药卫生—肿瘤]