β-环糊精-二次微分简易示波伏安法测定苯巴比妥  

2nd DERIVATIVE OSCILLO-VOLTAMMETRIC DETERMINATION OF PHENYLBARBITAL BY THE FORMATION OF INCLUSION COMPLEX WITH β-CYCLODEXTRIN (β-CD)

作　　者：陶福芳[1] 郑建斌[1] 

机构地区：[1]西北大学分析科学研究所,西安710069

出　　处：《理化检验（化学分册）》2003年第11期633-636,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助项目 ( 2 0 2 75 0 3 0 ) ;教育部科学技术研究重点项目 ( 0 2 87) ;教育部高等学校骨干教师资助计划项目 (GG 15 0 710 0 6 2 877);陕西省教育厅专项科研基金资助项目 ( 0 1JK0 76)

摘　　要：在NaOH底液中 ,β 环糊精能够产生灵敏切口 ,且该切口深度随苯巴比妥 β 环糊精包结物量的增加而变浅。根据 β 环糊精的示波特性 ,建立了用二次微分简易示波伏安法间接测定苯巴比妥注射液中苯巴比妥含量的新方法。苯巴比妥的浓度在 2 .5× 10 - 6 ～ 4 .5× 10 - 5mol·L- 1范围内的回归方程为h(V) =4 .5 5 9- 1.381× 10 4 c(mol·L- 1) ,相关系数为 - 0 .9976 ,检出限为 1.5×10 - 6 mol·L- 1。对于 2 .5× 10 - 5mol·L- 1苯巴比妥 5次测定的相对标准偏差为 2 .7% ,回收率为10 2 .1%。与高效液相色谱和其他方法相比 ,此法具仪器简单、简便快速。It was found that in a 0.2mol·L -1 NaOH solution, a sensitive incision was observed in the oscillovoltammogram caused by the presence of β CD, and that when phenylbarbital was present together with β CD, an inclusion complex was formed leading to a decrease of depth of the incision, which is in linear relationship with the concentration of phenylbarbital in the range of 2.5×10 -6 ～4.5×10 -5 mol·L -1 . Detection limit of this reaction was found to be 1.5×10 -6 mol·L -1 for phenylbarbital. The regression equation and correlation coefficient obtained were as follows: h(V) =4.559-1.381×10 4 c ( r =-0.9976) where h is the depth of incision and c is the concentration of phenylbarbital expressed in mol·L -1 . Precision was tested on the phenylbarbital concentration level of 2.5×10 -5 mol·L -1 ( n =5) and RSD obtained was 2.7%. Average recovery obtained was 102.1% ( n =5). As compared with the results found by HPLC, good conformity was obtained.

关 键 词：示波伏安法 苯巴比妥 二次微分 Β-环糊精 

分 类 号：R917[医药卫生—药物分析学] O657.1[医药卫生—药学]