急性混合性白血病细胞化学染色特点分析  

ANALYSIS OF CHARACTERISTICS WITH REGARD TO CYTOCHEMICAL STAINING IN PATIENTS WITH ACUTE HYBRID LEUKEMIA

作　　者：崔雯[1] 许议丹 孙万臣[1] 王建祥[1] 竺晓凡[1] 韩明哲[1] 钱林生[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,血液病医院,天津300020

出　　处：《白血病．淋巴瘤》2003年第6期323-325,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 :应用细胞化学染色诊断急性混合性白血病 (AHL)。方法 :1 0种细胞化学染色方法。结果 :AHL POX和 SBB可表现阳性 ,但阳性率均较低 ,以 <1 0 %为主。PAS染色可呈粗颗粒、珠状和块状或部分细胞呈细颗粒 ,部分粗颗粒、小珠。双克隆型髓系粒细胞和单核细胞的鉴别主要观察 POX阳性物分布及单核反应较强的 NSE、NBE、ACP。结论 :AHL是淋巴细胞和髓细胞共同累及的一组疾病 ,单纯形态学诊断准确率为 8.7% ,结合细胞化学染色可提高到 5 5 .6 %。POX、SBB和 CE主要表达髓细胞 ,5 0 % AHL 可以表达阳性 ,PAS染色主要表达淋系特点 ,二者结合可初步诊断 AHL,再结合POX酶型及单核反应强的酯酶染色 。Objective: To investigate the utilities of cytochemical staining technique in diagnosis of acute hybrid leukemia (AHL) . Methods: Ten kinds of cytochemical stains were performed . Results:There were faintly positive for POX and SBB stain in AHL patients , predominantly less than 10 % . For PAS staining, it was appeared as coarse granules , beads and blocks like , or mixture of diffuse fine granules and coarse granules and beads in some cells . In bi clonal type AHL patients with myeloid markers, The differentiations between granulocytes and monocytes depend on the distribution of POX positive materials and status of NSE, NBE, ACP that had strong reaction to monocytes.Conclusion : AHL is a group of diseases characterized by mixture of lymphocytes and myeloid cells . 8.7 percent of leukemia patients can be diagnosed as AHL based on morphological features, and 55.6 % of such patients would be determined when combined with cytochemical staining. POX, SBB and CE staining are mainly positive in myeloid cells, with a 50 % expression in AHL, and PAS is used to identify the lymphoid lineage. A preliminary diagnosis may be established by combination of these staining techniques, furthermore, the diagnosis is refined according to the type of POX staining and some esterase reacted strongly to monocytes.

关 键 词：诊断 化学染色 AHL 急性混合性白血病 染色方法 

分 类 号：R733.71[医药卫生—肿瘤]