骨肉瘤患者外周血淋巴细胞体外相关瘤细胞致敏和rIL-2诱导的杀伤细胞  

STUDY ON KILL CELLS FROM OSTEOSARCOMA PATIENTS STIMULATED BY INACTIVE TUMOR CELLS AND SUBSEQENTLY CULTURED WITH rIL-2

作　　者：高苏宁[1] 陈长玉[1] 沈晓秋[1] 黄攸生[1] 方振东[1] 赵红军[1] 邹晓波[1] 韩晓光[1] 

机构地区：[1]解放军第81医院骨科,南京210002

出　　处：《临床肿瘤学杂志》1996年第3期19-22,共4页Chinese Clinical Oncology

摘　　要：本实验对骨肉瘤患者外周血淋巴细胞(PBL)采用2种培养方法:(1)PBL在体外用患者肿瘤组织学相关传代骨肉瘤细胞(灭活处理)刺激5天再经rIL-2诱导12天;(2)单独rIL-2,激活培养PBL3～5天;分别获取rIL-2诱导体外刺激细胞毒淋巴细胞(rIL-2-CL)和LAK细胞。借助^(51)Cr释放试验对2种效应细胞形成及体外抗瘤能力进行比较性研究。结论:rIL-2-CL主要是一种特异性较高,杀伤活性强烈CTL细胞群,对抗LAK细胞的骨肉瘤细胞具有明显杀伤效应。In the study the two culture methods were used in that peripheral blood lymphocytes (PBL) from the patients with osteosarcoma were stimulated in vitro with inactive osteosarcoma cells for 5 days, and then induced rIL-2 for 12 days or only with rIL-2 for 4 days in ordor to develop rIL-2-CL(mixed lymphocyte tumor culture-stimulated cytotoxic lymphocytes induced with rIL-2) and LAK cells seperatively. The results indicates that rIL-2-CL were advantage over LAK cells in the specificity、duration of anti-osteosarcoma cells and the lysis of normal tissue, which might be more promising in the treatment ofosteosarcoma patients than LAK cells.

关 键 词：骨肉瘤 外周血淋巴细胞 瘤细胞 PBL RIL-2 杀伤细胞 治疗 

分 类 号：R738[医药卫生—肿瘤]