紫外线诱变康宁木霉提高酶活力的研究  

The study of improving the activity of Trichoderma koningii by ultraviolet mutagenesis

作　　者：李爱华[1] 李耀忠[2] 宋莉莉[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学后勤管理处,宁夏银川750021

出　　处：《宁夏大学学报（自然科学版）》2003年第4期389-391,共3页Journal of Ningxia University(Natural Science Edition)

基　　金：国家科技攻关计划项目(2002BA901A31);宁夏"十五"重点科技攻关项目(2001 004 02)

摘　　要：通过对康宁木霉菌种的诱变、筛选和固体发酵培养,研究了培养基、培养时间、诱变时间及pH值对诱变菌所产酶的活力的影响.结果表明,康宁木霉经诱变5min,在pH为6.0的玉米秸PDA培养基上培养72h,产生的C1酶和Cx酶的酶活力较高,C1酶的酶活力由73u/g提高到176u/g,增加了2.41倍,Cx酶的酶活力由798u/g提高到2069u/g,增加了2.59倍.Culture medium, culture time, induction time and medium pH were studied in this experiment about the effects on the activity of Trichoderma koningii-enzyme. The results showed that Trichoderma koningii was inducted for 5 min, then cultured for 72 h in the fodder medium that pH was 6.0, the activity of C_1 and C_x was higher than before, and the activity of C_1 was improved from 73 u/g to 176 u/g, which were improved by 2.41 times, the activity of C_x was improved from 798 u/g to 2 069 u/g, which were improved respectively 2.59 times.

关 键 词：紫外线 线诱变 康宁木霉 酶活力 康氏木霉 纤维素酶 

分 类 号：Q55[生物学—生物化学] Q936