Cloning of human brevican cDNA and expression of its mRNA in human glioma  

作　　者：韩晞 董艳 由振东 何成 卢亦成 

机构地区：[1]Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China [2]Department of Neurobiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China

出　　处：《Journal of Medical Colleges of PLA(China)》2003年第6期388-392,共5页中国人民解放军军医大学学报（英文版）

摘　　要：Objective: To clone the cDNA of human brevican secreting isoform and to investigate its mRNA expression in human glioma. Methods: The full-length cDNA of human brevican secreted isoform was cloned from a human anaplastic astrocytoma by RT-PCR, and the expression of human brevican mRNA in 22 cases of human glioma and 13 cases of non-glial brain tumors were investigated by in situ hybridization. Results: The cDNA which including the whole open reading frame of human brevican secreted isoform was obtained. In situ hybridization showed that brevican positive cells were present in all of the 22 cases of gliomas (100%), whereas none were found in the 13 cases of non-glial and metastasis brain tumors examined. Conclusion: The results suggest that brevican mRNA is highly and specifically expressed in human glioma.Objective: To clone the cDNA of human brevican secreting isoform and to investigate its mRNA expression in human glioma. Methods: The full-length cDNA of human brevican secreted isoform was cloned from a human anaplastic astrocytoma by RT-PCR, and the expression of human brevican mRNA in 22 cases of human glioma and 13 cases of non-glial brain tumors were investigated by in situ hybridization. Results: The cDNA which including the whole open reading frame of human brevican secreted isoform was obtained. In situ hybridization showed that brevican positive cells were present in all of the 22 cases of gliomas (100%), whereas none were found in the 13 cases of non-glial and metastasis brain tumors examined. Conclusion: The results suggest that brevican mRNA is highly and specifically expressed in human glioma.

关 键 词：BREVICAN RT-PCR in situ hybridization GLIOMA 

分 类 号：R73-3[医药卫生—肿瘤]