Anti-inflammatory and analgesic activities with gastroprotective effect of semi-purified fractions and isolation of pure compounds from Mediterranean gorgonian Eunicella singularis  

作　　者：Monia Deghrigue Carmen Festa Lotfi Ghribi Maria Valeria D’Auria Simona De Marino Hichem Ben Jannet Abderrahman Bouraoui 

机构地区：[1]Laboratoire de développement chimique,galénique et pharmacologique des médicaments (LR12ES09).Equipe de Pharmacologie marine,Faculté de pharmacie de Monastir,Université de Monastir [2]Department of Pharmacy,University of Naples "Federico II" [3]Laboratoire de chimie hétérocyclique,produits naturels et réactivité. Equipe de chimie médicinale et produits naturels (LR11ES39),Faculté des sciences de Monastir,Université de Monastir [4]Laboratoire de chimie hétérocyclique,produits naturels et réactivité.Equipe de chimie médicinale et produits naturels (LR11ES39),Faculté des sciences de Monastir,Université de Monastir

出　　处：《Asian Pacific Journal of Tropical Medicine》2015年第8期598-603,共6页亚太热带医药杂志（英文版）

摘　　要：Objective: To explore anti-inflammatory activities of organic extract and its semi-purified fractions(ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. Methods: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/Et OH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. Results: The fraction F-Et OH showed an important antiinflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. Conclusions: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.Objective: To explore anti-inflammatory activities of organic extract and its semi-purified fractions(ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. Methods: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/Et OH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. Results: The fraction F-Et OH showed an important antiinflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. Conclusions: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.

关 键 词：Eunicella singularis ANTI-INFLAMMATORY ACTIVITY ANALGESIC ACTIVITY GASTROPROTECTIVE effect Diterpenoid STEROLS 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]